Xll CONTENTS. 



CHAPTER III. 



MICROSCOPICAL EXAMINATION OF BACTERIA IN LIQUIDS, 

 IN CULTIVATIONS ON SOLID MEDIA, AND IN TISSUES. 



PAGE 



a. Examination in the fresh state . . . . -44 



b. Cover-glass preparations : Methods of Ehrlich, Babes, and His . 46 



c. Cover-glass impressions . . . . . -49 



CHAPTER IV. 

 PREPARATION AND STAINING OF TISSUE SECTIONS. 



a. Methods of hardening and decalcifying preparations . 51 



b. Methods of embedding, fixing and cutting . . . 52 

 f. General principles of staining bacteria in tissue sections : Methods 



of Weigert, Gram, and Weigert-Ehrlich . 54 



CHAPTER V. 



PREPARATION OF NUTRIENT MEDIA AND METHODS OF 



CULTIVATION. 

 SOLID MEDIA: 



a. Preparation of sterile gelatine-, and agar-agar-peptone-broth . 61 



b. Methods of employing nutrient jelly in test-tube- and plate-cultiva- 



tions ........ 66 



c. Preparation and employment of sterilised potatoes, potato-paste, 



bread-paste, vegetables, fruit and white of egg. * 77 



d. Preparation and employment of sterile blood serum " * . . 81 



LIQUID MEDIA : 



e. Preparation of sterilised bouillon, liquid blood serum, urine, milk, 



vegetable infusions, and artificial nourishing liquids . . 83 



/. Methods of storing and employing liquid media. Listers flasks, 

 Aitken's test-tubes, Sternberg's bulbs, Pasteur's apparatus, 

 Miquel's bulbs, Drop-cultures . . . . .86 



