72 BACTERIOLOGY. 



quickly replace the cover of the box. On removing 

 the plug from "the original," an assistant raises 

 the bell-glass, and the contents of the tube are 

 poured on to the plate ; with a glass rod the gelatine 

 must be then rapidly spread out in an even layer 

 within about half an inch of the margin of the 

 plate. The assistant replaces the bell-glass, and the 

 gelatine is left to set. Meanwhile a glass bench or 

 metallic shelf is placed in the damp chamber, ready 

 for the reception of the plate-cultivation, and when 

 the gelatine is quite solid the plate is quickly trans- 

 ferred from under the bell-glass to the damp chamber; 

 precisely the same process is repeated with tubes i 

 and 2. and the damp chamber, labelled with the 

 details of the experiment, is set aside for the colonies 

 to develop. Not only plate- cultures should be care- 

 fully labelled with date and description, but the 

 same remark applies equally to all preparations, 

 tube- cultures, potato-cultures, drop-cultures, etc. 

 In plate-cultivations write the source of the mate- 

 rial, the date, and the number of inoculations; for 

 example, thus : 



Finkler* s comma - bacilli. 



From tube-cultivation on " agar-agar," 5th Feb- 

 ruary, 1885. 



Lower plate (Orig.) . i ose from cultivation. 

 Middle plate, No. i . 3 osen from Orig. 

 Upper plate, No. 2 . .3 osen from NQ. i. 



