LIQUID MEDIA. 89 



each limb with a bent arm, is a convenient form for 

 storing sterilised bouillon. The sealed end of an 

 arm is nipped off with sterilised forceps, the sterile 

 bouillon aspirated into each limb, and the arm 

 again sealed in the flame ; a series of such tubes 

 can be arranged upon a rack on the working 

 table.* 



Bulbs with a vertical neck drawn out to a fine 

 point ; others with a neck bent at an obtuse angle 

 plugged with cotton wool, and a lateral curved arm 

 drawn out to a fine point, are also employed. For 

 a description of these various vessels and their 

 special advantages the works of Pasteur and 

 Duclaux must be consulted. 



Miquel's Bulbs. The lube a boule of Miquelf 

 is also a very useful form. It consists of a bulb 

 of 50 cc. capacity blown in the middle of a glass 

 tube. The part of the tube above the bulb is con- 

 tracted about half way between the bulb and its 

 extremity, and can either be left quite straight or 

 can be made to curve slightly. On either side of the 

 contraction the tube is plugged with asbestos. The 

 portion of the tube below the bulb is S shaped, and 

 drawn out at its extremity into a fine point. The 

 bulbs are charged with nutrient liquid and inocu- 

 lated by aspiration, and the point of the S tube 

 sealed in the flame of a Bunsen burner. 



Drop-Cultures. This method of cultivation 



* Duclaux, Ferments et Maladies. 1882. 



f Miquel, Les Organismes Vivants de T Atmosphere. 1883. 



