CHAPTER VII. 



EXAMINA TION OF ANIMALS EXPERIMENTED UPON 

 AND THE METHODS OF ISOLATING MICRO- 

 ORGANISMS FROM THE LIVING AND DEAD 

 SUBJECT. 



METHOD OF DISSECTION AND EXAMINATION. 



ALL animals that die after an experimental inocu- 

 lation should be examined immediately after death. 

 Every precaution must be taken, in conducting 

 the dissection, to exclude extraneous micro-organ- 

 isms, and all instruments employed must have been 

 sterilised in the hot-air steriliser, or heated in the 

 Bunsen burner. If a mouse, for example, has died 

 after an inoculation, it should be at once pinned 

 out by its feet on a slab of wood or in a gutta- 

 percha tray, and bathed with sublimate solution. 

 In the same way, before examining a dead rabbit, a 

 stream of sublimate should be directed over it to 

 lay the fur, which otherwise interferes with the 

 dissection. The hair should be cut away with 

 sterilised scissors from the seat of inoculation, which 

 is the first part to be examined, and any suppura- 

 tion, haemorrhage, oedema, or other pathological 



