EXAMINATION OF ANIMALS EXPERIMENTED UPON. 99 



Having completed the dissection, the organs of 

 such a small animal as a mouse may be removed 

 en masse and transferred to absolute alcohol for 

 subsequent examination. In other cases it may be 

 only necessary to reserve portions of each organ. 

 In any case it should be remembered that with a 

 virulent micro-organism, e.g., anthrax, any remain- 

 ing part of the animal should be cremated, and the 

 hands and all instruments should be thoroughly 

 disinfected. 



Isolation of Micro-organisms from the 

 Living Subject. Micro-organisms in the living 

 subject may be isolated from pus of abscesses, 

 or other discharges, and from the blood and tissues. 

 Abscesses should be opened, and other operations 

 performed, when practicable, with Listerian precau- 

 tions, and a drop of the discharge taken up with an 

 ose or capillary pipette as already explained. 



To make a cultivation from the blood of a living 

 person, the tip of a finger must be well washed with 

 soap and water and bathed with strong sublimate, 

 or i in 20 carbolic, solution. Venous conges- 

 tion is produced by applying an elastic band 

 or ligature to the finger, which is pricked with a 

 sterilised sewing needle. From the drop of blood 

 which exudes the necessary inoculations and ex- 

 aminations can be made. 



