SYSTEMATIC AND DESCRIPTIVE, 141 



(a) Koch's method.* 



Sections of the intestine, which must be well hardened 

 in absolute alcohol, are left for twenty-four hours in 

 a strong watery solution of methylene-blue, or for a 

 shorter time if the colour solution is warmed. Then 

 treated in the usual way. 



(b) Babes method.-^ 



Sections, preferably from a recent case of cholera, and 

 made as soon as possible after death, are left for 

 twenty-four hours in a watery solution of fuchsine 

 (fabrique de Bale), then washed in distilled water 

 faintly acidulated with acetic acid, or in sublimate 

 solution (i 1000), passed rapidly through alcohol 

 and oil of cloves, dried with filter paper, and pre- 

 served in Canada balsam. 



(c) Nicati and Rietsctts method*. 



A small quantity of the stools or of the scraping of the 

 intestinal mucous membrane is spread out on a 

 glass slide and dried, then steeped during some 

 seconds in sublimate solution or in osmic acid 

 (i 100). It is then stained by immersion in 

 fuchsine-aniline solution (i or 2 grammes of Bale 

 fuchsine dissolved in a saturated aqueous solution 

 of aniline), washed, dried, and mounted in Canada 

 balsam. 



Spirillum Finklerii (Commi-baciilus of Cholera 

 nostras}. Curved rods thicker than the comma- 

 bacillus of Koch, and spirilla. The colonies on 

 plate cultivations (Plates VI. and VII.) are very 

 much larger than those of the comma- bacillus 



* Berliner Klinische Woch., No. 31. 



f Cornil and Babes, Les Bacteries, p. 458, 1885. 



J Brit. Med. Journal, Sept. 1885. 



