148 BACTERIOLOGY. 



(b) Pour off the extract, and dilute it to the 

 Sp. Gr. 1*004. 



(c) Boil gently for one hour in a bulb plugged 

 with cotton wool. 



(d) Set aside 500 ccm. of the extract at 36C. 



In about twenty-four hours, as a rule, a pellicle 

 has commenced to develop upon the surface of 

 the liquid. If the reaction is definitely acid, 

 carbonate of soda solution must be added to the 

 decoction. 



METHODS OF STAINING HAY BACILLUS. 



To demonstrate the flagella of the bacilli, they must be 

 stained with haematoxylin solution (Koch). 



The linking together of cocci, long rods, and short rods 

 in the threads, is shown by treating with alcoholic solution 

 of fuchsine, or with iodine solution (Zopf). 



To stain the spores the cover-glass preparations must be 

 heated to a very high temperature (210 C.), in the hot-air 

 steriliser for half an hour, or they may be exposed for a 

 few seconds to the action of concentrated sulphuric acid 

 (Buchner). 



Bacillus anthracis (Bactiridie du charbon, Bacil- 

 lus of splenic fever, woolsorterf disease, or malignant 

 pustule). Rods, 5 20 JJL long and i 1*25 //, broad, 

 and threads, made up of rods and cocci. As a 

 thorough knowledge of the life-history of this 

 bacillus is of the greatest importance, inasmuch as 

 it is without any doubt the actual cause of wide- 

 spread disease, the various steps to be followed 



