SYSTEMATIC AND DESCRIPTIVE. 153 



and staining them with aniline dyes. The filaments, 

 examined with a high power, will then be seen to 

 consist of a number of rods or segments (Plate I., 

 Fig. 30). On the other hand, filaments from a 

 tube cultivation in a solid medium will be found 

 to be composed, not only of rods, but here and 

 there of torula-like involution-forms (Plate I., Fig. 

 30). , In a cover-glass-impression from a potato- 

 culture (Plate I., Fig. 29) the individual segments 

 have a great tendency to be isolated one from the 

 other, and there is copious spore-formation. 



Preservation of spores. Spores may be preserved 

 simply by allowing anthracic blood to dry and 

 sealing it in a tube. The spores from a potato 

 cultivation are treated as follows : The inocu- 

 lated surface containing the creamy cultivation 

 is sliced off in a thin layer, and is mashed up 

 with distilled water in a glass capsule. Sterilised 

 silk-thread is cut up into lengths of about a quarter 

 of an inch, and allowed to soak in the paste for 

 some hours, under a bell-glass. The threads are 

 then picked out with a pair of forceps, and laid upon 

 a sterilised glass plate, covered with a bell-glass, 

 and allowed to dry. From the plate, when perfectly 

 dry, they are transferred to a small test-tube, which 

 can be plugged with cotton-wool, or sealed in the 

 Bunsen burner. 



Examination in the tissues. The organs must be 

 hardened in absolute alcohol, cut and stained, 

 (pp. 51, 157). The method of Gram is the most 



