154 BACTERIOLOGY. 



instructive, and eosin a very satisfactory contrast 

 stain. The capillaries all over the body, lungs, 

 liver, kidney, spleen, skin, mucous membrane, etc., 

 will be found to contain bacilli. In some cases the 

 bacilli are so numerous, (e.g., in the capillaries of 

 the kidney, Plate XVI., Fig. 2), that examination 

 with a low power gives the appearance of an 

 injected specimen. 



Inoculation of animals. A thread containing spores, 

 a drop of blood from an infected animal, or a 

 minute portion of a cultivation, introduced under 

 the skin of a mouse or guinea-pig, causes its death, 

 as a rule, in from twenty-four to forty-eight hours. 

 Sheep fed upon potatoes which have been the 

 medium for cultivating the bacillus, die in a few 

 days. Goats, hedgehogs, sparrows, cows, horses, 

 are all susceptible. Rats are infected with difficulty. 

 Pigs, dogs, cats, white rats, and Algerian sheep 

 have an immunity from the disease. Frogs and 

 fish have been rendered susceptible by raising the 

 temperature of the water in which they lived. 



Dissemination of the disease and mode of infection. It 

 has been stated that when carcases of animals which 

 have died of anthrax are buried under the soil, the 

 development of the bacilli into spores can take place. 

 The spores were supposed to be taken up by earth 

 worms, carried to the surface, and deposited in their 

 castings ; animals then grazing or sojourning on 

 the soil are thus liable to be infected.* This has not 



* Pasteur, Bulletin de r Academic de Medecine. 1880. 



