174 



BACTERIOLOGY. 



mune. Rabbits inoculated in the cornea suffer 

 from an intense inflammation of the eyes. The 

 bacilli are easily cultivated outside the body on 

 a mixture of aqueous humour and gelatine, and 

 especially on nutrient gelatine rendered slightly 

 alkaline with sodium phosphate. They grow also 



very well on the ordi- 

 nary nutrient gelatine, 

 forming in plate cul- 

 tivations scarcely per- 

 ceptible cloud-like 

 specks, and in a test- 

 tube of nutrient 

 gelatine they form a 

 delicately clouded cul- 

 tivation along the 

 needle track (Figs. 38 

 and 39). A small 

 quantity of a pure 

 cultivation carried 

 through many gene- 

 rations reproduces 

 the disease when in- 

 oculated into mice. 

 The organs should be 

 hardened in absolute 

 alcohol, and sections 

 stained preferably by the method of Gram (Plate 

 XXII., Figs, i and 2). 



Bacillus typhosus, Eberth (Bacillus of typhoid 



Fig. 38. Fig. 39. 



PURE-CULTIVATIONS OF THE BACILLUS 

 OF SEPTICAEMIA OF MICE IN GELA- 

 TINE-PEPTONE-BROTH. 



Fig. 38. In two days. 



Fig. 39. In five days. 



