SYSTEMATIC AND DESCRIPTIVE. 177 



METHODS OF STAINING THE BACILLUS OF LEPROSY. 



Cover-glass-preparations may be made in the ordinary 

 way, or by a special method, which consists in clamping a 

 nodule with a pile-clamp, until a state of anaemia of the 

 tissue is produced. On pricking with a needle or sharp 

 knife a drop of clear fluid exudes, from which cover-glass- 

 preparations may be made.* Cover-glass-preparations 

 and sections may be stained by Ehrlich's method (p. 48), 

 or the latter by the following process : 



Method of Babes.\ Preparations are stained in a solution 

 of rosaniline hydrochlorate in aniline-water. Decolorise in 

 33 per cent, hydrochloric acid, and after-stain with methy- 

 lene-blue. 



Bacillus of diphtheria of man. Rods about 

 the same length as the tubercle bacillus, but about 

 twice as thick ; the longer ones consist of single 

 individuals linked together. Cultivated in 5 per 

 cent, gelatine at 20 22 C., the rods develop into 

 irregular involution-forms. Inoculation results are 

 doubtful. The bacillus was isolated from diph- 

 theritic membrane. J In the diphtheria of calves and 

 pigeons a bacillus also has been observed. Inocu- 

 lation with a cultivation of the latter produced the 

 disease in young pigeons and sparrows. (Vide 

 Streptococcus diphtheriticus^) 



Bacillus malariae, Klebs (Bacillus of inter- 

 mittent fever]. Rods, 2 7 \L long, which grow 

 into twisted threads. Spore formation takes place 



* Manson, l^ancet. 1884. 



f Compt. Rend, de rAcadd. Sc. 1883. 



+ Loffler, Mittheil' a.d. Gesundheitsamt, Zweiter Band. 



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