I 7 8 BACTERIOLOGY. 



in the centre, or at either end (Plate I., Fig. 14). 

 They grow in liquids rich in nitrogen, such as 

 albumen, urine, and glue. Deprived of oxygen, 

 they cease to develop, and are, therefore, aerobic. 



Inoculated into rabbits they produce a febrile 

 disorder considered analogous to malarial fever,* 

 and in the spleen and marrow the threads and 

 spores of the bacilli were found in abundance. 

 Bacilli with end-spores have been discovered also 

 in the blood of patients suffering from malaria. f 



The bacillus was first described as present in the 

 soil of the Roman Campagna. 



Bacillus mallei (Bacillus of glanders). Rods 

 about the size of tubercle bacilli. When cultivated 

 on solid sterile blood serum at 38 C., the growth 

 appears in the form of minute, transparent drops 

 consisting entirely of the characteristic bacilli. On 

 sterilised potatoes they form, in a week to ten 

 days at 37 C., a brown gelatinous layer. Pure 

 cultivations after several generations produce the 

 following results when inoculated into horses, rab- 

 bits, guinea- pigs, and field-mice. A spreading ulcer 

 with indurated base appears at the site of inocula- 

 tion, while smaller ulcers break out in its vicinity. 

 The lymphatics become swollen, and general in- 

 fection follows in the form of nodules in the internal 

 organs, and nodules and ulcers on the nasal septum. 

 In guinea-pigs a characteristic tumour of the testis, 



* Klebs and Tommasi-Crudeli, Archiv f. Exper. Pathol., 1879. 

 t Marchiafava, ibid. 



