SYSTEMATIC AND DESCRIPTIVE. 179 



or ovary and vulva, frequently results, and should be 

 prepared for microscopical sections. The bacilli are 

 found in the nodules of the nasal mucous mem- 

 brane, the lung, spleen, liver, and other organs in 

 horses and sheep affected with glanders. 



METHODS OF STAINING THE BACILLUS OF GLANDERS. 



The bacilli of glanders are extremely difficult to demon- 

 strate. The most satisfactory results are obtained as follows : 



Method of Schiitz. The sections are placed for twenty- 

 four hours in a mixture of 



Potash solution (i in 10,000); ) Equal 



Concentrated alcoholic methylene-blue soltn. ; j parts. 



Wash the sections in a watch-glass with water acidulated 

 with four drops of acetic acid. Transfer for five minutes 

 to 50 per cent, alcohol, fifteen minutes to absolute alcohol, 

 clarify in clove-oil, and mount in Canada balsam. 



Bacillus caucasicus, Kern. Rods forming 

 two spores, one at each end, otherwise similar to 

 bacillus subtilis. They occur in the form of whitish 

 lumps in company with Saccharomyces mycoderma in 

 the production of a drink " kephir " from cow's 

 milk. The fermentation is not due to the bacillus. 



Bacillus dysodes, Zopf. Cocci, long and short 

 rods, and spores. They were observed in bread, 

 making it greasy and unfit for food, and generating 

 a penetrating odour resembling a mixture of pep- 

 permint and turpentine. A great loss may result 



