2O8 BACTERIOLOGY. 



METHOD OF STAINING THE BACILLI OF ULCERATIVE 

 STOMATITIS. 



Sections through the ulcerations of the calf's tongue, or 

 of the inoculated tissue of the rabbit, were stained by 

 immersion in a mixture of magenta and methylene-blue. 

 They were then washed in spirit, cleared in clove-oil, and 

 mounted in Canada balsam. 



Bacillus of syphilis, Lustgarten.* Rods re- 

 sembling the bacilli of leprosy and tuberculosis, 

 3 4 JJL long, '8 p thick. Two or more colourless, 

 ovoid points in the course of the rod are visible with 

 a high power ; it is thought that they are possibly 

 spores. The bacilli are always found in the interior 

 of nucleated cells which are more than double the 

 size of leucocytes. They have been observed in the 

 discharge of the primary lesion, and in hereditary 

 affections of tertiary gummata. 



METHOD OF STAINING THE BACILLUS OF SYPHILIS. 



Method of Lustgarten : 



Sections are placed for from twelve to twenty-four hours 

 in the following solution, at the ordinary temperature of the 

 room, and finally the solution is warmed for two hours 

 at 60 C. :- 



Concentrated alcoholic solution of gentian-violet 1 1 

 Aniline water . 100 



The sections are then placed for a few minutes in ab- 

 * Lustgarten, Die Syphilisbacillen. Mit 4 Tafeln. 1885. 



