SPECIES DESCRIBED BY VARIOUS WRITERS. 2IQ 



glass must then be gently pressed down upon the prepara- 

 tion, which is then examined. To stain the fresh tufts, the 

 little fungus masses are teased out, and transferred to a 

 watch-glass containing alcohol, to which a few drops of 

 concentrated alcoholic solution of eosin are added. They 

 can then be mounted and preserved in glycerine. 



Sections can be stained by either of the following 

 methods. 



Weigert's Method: 



Immerse the sections for one hour in orseille, rinse with 

 alcohol, and after-stain with gentian- violet (Plate XXVIII.). 



Plants Method :- 



Sections are left for ten minutes in Gibbes' solution (No. 

 22), warmed to 45 Q C. They are then rinsed in water, and 

 after-stained in concentrated alcoholic solution of picric 

 acid for five to ten minutes; immersed in water five minutes, 

 50 percent, alcohol fifteen minutes, passed through absolute 

 alcohol and clove-oil, and preserved in Canada balsam 

 (Plate XXVII.). 



