Explanation of Plate 40. 



BACILLUS ANTHRACIS. F. Colin and E. Koch. 

 (Anthrax.) 



I. Smear preparation from the blood of a mouse's 



spleen, x 1,000. 



II. Impression preparation from agar plate culture, 

 one day at 22. x 1,000. 



III. Unstained preparation in hanging drop from bouil- 



lon culture, thirty -six hours at 37. Spores 

 beginning to drop out. X 1,000. 



IV. Anthrax threads from agar, thirty-six hours at 



37. Stained with Ziehl's solution. Spores 

 red, bacilli blue, x 1, 000. 

 V. Involution forms, five weeks old, from agar. 



Stick culture stained with fuchsin< x 1,000. 

 VI. Unstained preparation in hanging drop from 

 bouillon culture, eight hours at 37. Begin- 

 ning of sporulation. x 1, 000. 



40 



