Explanation of Plate 45. 



BACILLUS TETANI. Nicolaier. 

 (Tetanus bacillus.) 



I. Sugar-agar stick culture, three days at 37. 

 II. Sugar-gelatin stick culture, six days at 22. 



III. Sugar-gelatin plate, four days at 22. Natural 



size. Cultivated anaerobic. 



IV. Sugar-gelatin plate, four day sat 22. x60. Su- 



perficial and deep colony. Cultivated ana- 

 erobic. 

 V. Sugar-agar plate, four days at 37. Natural size. 



Cultivated anaerobic. 



VI. Sugar-agar plate, four day sat 37 , x60. Super- 

 ficial and deep colony. Cultivated anaerobic. 

 VII. Microscopic preparation. Pure culture from 

 sugar-agar, three days at 37. x 1,000. 

 Bacilli with spores. Ziehl's double stain. 

 VIII. Microscopic preparation. Pure culture from 

 sugar-agar, two days at 37. x 1,000. A few 

 bacilli with spores. Fuehsin stain. 

 IX. Microscopical preparation. Pure culture from 

 sugar-agar, twenty -four hours at 37 . x 1, 000. 

 Extremely long filaments with faintly colored 

 interspaces. 



X. Microscopical preparation. Pure culture from 

 sugar-agar, six days at 37. x 1,000. Fuchsin 

 stain. Long filaments and spore chains with 

 faintly colored interspaces. 



