MOEPHOLOGY OF BACTERIA. 



79 



the bacillus at right angles to the direction of the fila- 

 ment is rarely observed (vide Sorokin, C. B., I., 465). 



The following cut shows the germination of a few 

 closely allied varieties which were studied by L. 

 Klein. 



The examination is made in a hanging drop of gel- 

 atin or agar. This may furnish very valuable mate- 



FIG. 10. Development of Spores, according to L. Klein, a, Bacillus 

 leptosporus L. Klein; 1-3, the enlarging spore; 4, the spore is con- 

 verted into the bacillus without any sharp demarcation ; 5-10, further 

 growth; 11-16, development of the spores. 6, Bacillus sessilis L. Klein; 

 1-4, the spore swells; 5, the spore sends out a little rod at one pole, 

 and remains behind as an empty envelope; 6-8, further growth; 9-13, 

 development of the spores. 



rial in differential diagnosis, as it seems to differ 

 greatly in details. 



1. Bacillus anthracis. The spore swells, its refrac- 

 tive power diminishes, its sharply defined membrane 

 becomes indistinct, and without any sharp demarca- 

 tion the spore becomes a young bacterium cell, which 

 grows further and divides again. 



A similar condition obtains in the bacillus lepto- 

 sporus Klein, described by L. Klein (0. B., VI., 377), 

 which is characterized by narrow, almost quadrangu- 

 lar spores (Fig. 10, a). 



