86 



ATLAS OF BACTERIOLOGY. 



ful studies. The energy necessary to the albumin 

 synthesis seems to be gained by oxidation of ammo- 

 nia into nitric acid. 



Very few practically important bacteria exhibit 

 such simplicity, but very many can dispense at least 

 with albumin in the nutrient and thrive in solutions 

 of very simple composition. Formerly cultures in 

 such fluids were employed very often, and recently 

 Uschinsky has again resorted to simple nutrients. 

 But instead of Uschinsky 's somewhat complicated so- 

 lution : 



Water 1,000 



Glycerin 30-40 



Sodium chloride 5-7 



Calcium chloride. . 0.1 



Magnesium sulphate . . . 0.2-0.4 



Dikalium phosphate . . . 3-2. 5 



Ammonium lacticum . . . 6-7 



Sodium asparaginicum . 3-4 



we may choose much simpler solutions ; for example, 

 on the recommendation of Voges and C. Fraenkel 

 (Hyg. Bundschau, 1894, No. 17) for one litre: 



Sodium chloride 5 gm. 



Neutral commercial sodium phosphate 2 gm. 



Ammonium lactate 6 gm. 



Asparagin 4 gm. 



In this fluid (although it contains no sulphur) there 

 grow: 



VERY WELL. 



Bacillus subtilis and mycoides. 



Bacterium syncyaneum, pyocyaneum, coli, acidi lactici, 

 pneumonia), mallei, vulgare. All vibriones. 



FEEBLY. 



Micrococcus pyogenes a 



aureus. 



Streptococcus pyogenes. 



Bacterium typhi. 



Bacillus anthracis. 



