TECHNICAL APPENDIX. 189 



lye; place in it the infected stick culture and close 

 tlie cylinder at once with a soft-rubber stopper or a 

 ground-glass stopper which is sealed with paraffin. 

 According to Kitasato the anaerobics which are less 

 sensitive to oxygen may be cultivated in saccharine 

 agar in a high stick culture, even without pyrogallic 

 acid. A wire with a small loop is pushed into the 

 layer of sugar agar (8 to 10 c.c. high), and the wire 

 turned on its long axis before withdrawal. 



(b) For plate cultures we use, instead of the glass 

 cylinder, a wide exsiccator with a ground cover ; fill 

 the lower part with sand and the pyrogallic-acid mix- 

 ture, and then manipulate as before. 



III. EXPERIMENTS ON ANIMALS. 

 A. Infection. 



1. Subcutaneous inoculation. A shallow incision 

 is made with a pair of scissors on some part of the 

 skin, after it has been washed with a 0.1-per-cent 

 solution of corrosive sublimate; the inoculating 

 matter is carried beneath the skin by means of a 

 stout platinum wire with a loop. Mice are generally 

 inoculated above the root of the tail ; they are simply 

 held by the tip of the tail, and allowed to hang into a 

 glass which is covered up in great part by a piece 

 of wood. Guinea-pigs and rabbits are inoculated on 

 the sido of the thorax. 



2. Subcutaneous injection is generally effected by 

 means of Koch's rubber ball injection syringe or 

 Strohschein's syringe. A fold of skin is picked up 

 at some part of the body, and the needle inserted in 

 the longitudinal direction. If several cubic centi- 



