190 ATLAS OF BACTERIOLOGY. 



metres are to be injected, the following simple method 

 may be adopted: A short piece of rubber tube pro- 

 vided with an injection needle is fastened to a grad- 

 uated pipette, the entire apparatus sterilized, the 

 pipette filled, and the fluid blown' in by the aid of the 

 mouth or a rubber bulb. 



3. Peritoneal injection is made by perforating with 

 a sterilized hollow canula, at one puncture, the ab- 

 dominal wall, then cautiously advancing the needle 

 and injecting the fluid. 



B. Observation. 



Mice may be kept in sterilized glass vessels closed 

 with cotton and wire netting ; larger animals must be 

 kept in sterilized cages or stalls. 



C. Autopsy and Disposal of the Cadaver. 



Autopsies must be made immediately after death, 

 or, at least, the animal placed on ice. The animal, 

 lying on the back, is tied or nailed through the legs 

 to a board, the abdomen and chest are throughly 

 moistened with corrosive sublimate, and then the ab- 

 dominal cavity is opened with a previously sterilized 

 knife. The abdominal walls are separated and from 

 the spleen, liver, and kidneys some blood (or tissue 

 juice) is removed with a sterilized platinum loop. 

 This is smeared at once upon prepared agar plates. 

 The organs are carefully cut out, avoiding contact 

 with the intestines, and are placed in absolute alcohol 

 for further examination. Then the thorax is opened 

 with a pair of scissors, blood taken from the heart and 

 lungs, and these organs are placed in alcohol. Be- 

 fore each operation the instruments must be carefully 



