Die Tecliiiik der (jewebskiiltur in vitro. 



Von Alexis Carrel und Moutrose T. Burrows, New York. 



(Aus dem Laboratorium des Rockefeiler Institutes für medizinische Forschung.) 



Man kultiviert ein Gewebe oder ein Organ, indem man unter ge- 

 wissen Bedingungen Fragmente dieses Gewebes oder Organes in einen 

 passenden Kulturnährboden einimpft. Diese neue Methode, deren Resultate 

 zum Teil schon beschrieben worden sind ^), ist zahlreicher Anwendung fähig. 



*) Barrison, Observations ou livin,"- developiug nei've fibres. l'roc. of the Soc. for 

 Exp. Biolog. and Med. 1907. p. 140. — Embryonic trausplantation and the development of 

 the nervous System, the Harvey lectures 1907—1908. — The outgrowth of the nerve fibre as 

 a mode of protoplasmic movement. Journal Exp. Zool. 1910. Vol. 9. p. 787. — Burrows, The 

 growth of tissues of the chick embryo outside of the animal body with special reference to 

 the nervous System. Journal of Exp. Zool. 1911. Vol. 10. p. 63. — Culture des tissus d'embryon 

 de poulet et specialement culture des nerfs de poulet en dehors de l'organisme. C. R. Soc. de 

 Biologie. 1910. p. 291. — Carrel and Burrous, Cultivation of adult tissues and organs out- 

 side of the body. Journ. Am.Med. Assoc. 1910. p. 1379. — Cultivation of sarcoma outside of 

 the body. Journ. Am. Med Assoc, 1910. p. 1554. — Human sarcoma cultivated outside of 

 the body. Journ. Am. Med. Assoc. 1910. p. 1732. — Artificial Stimulation and iuhibition 

 of the growth of normal and sarcomatous tissues. Journ. Am. Med. Assoc. 1911. p. 32. — 

 Cultivation of tissues in vitro and its technique. Journal of Exp. Med. 1911. Vol. 13. 

 p. 387. — Cultivation in vitro of the thyroid gland. Journ. of Exp. Med. 1911. Vol. 13. 

 p. 416. — On the physico-chemical regulation of the growth of tissues. Journ. of Exp. 

 Med. 1911. Vol. 13. p. 562. — Cultivation in vitro of malignant tumors. Journ. of Exp. 

 Med. 1911. Vol. 13. n. 571. — La culture des tissus adultes en dehors de Forganisme. 

 C. R. Soc. de Biol. 1910. p. 293. — Culture de substance renale en dehors de l'organisme. 

 C. R. Soc. de Biol. 1910. p. 298. — Culture de moelle osseuse et de rate. C. R. Soc. 

 de Biol. 1910. p. 299. — Cultures primaires. secondaires et tertiaires de glande thyroide 

 et culture de peritoine. C. R. Soc. de Biol. 1910. p. 329. — Culture de sarcome en dehors 

 de l'organisme. C. R. Soc. de Biol. 1910. p. 332. — Culture in vitro d'un sarcome huniain. 

 C. R. Soc. de Biol. 1910. p. 367. — Seconde generation de cellules thyroidicnnes. C. R. 

 Soc. de Biol. 1910. p. 365. — A propos des cultures „in vitro" des tissus de mammi- 

 feres. C. R. Soc. de Biol. 1911. p. 3. — La culture des tissus in vitro. La Presse Medi- 

 cale. 1911. p. 209. — Carrel, Abnormal forms of life and their applications. American 

 Philosophical Society, Philadclpliia 1911. — Biifh, Cicatrisatiou of wounds in vitro. 

 Journ. of Exp. Med. 1911. Vol. 13. p. 422. — The influence of distilled water ou the 

 healing of skin wounds in the frog. Jouru. of Exp. Med. 1911. Vol. 13. p. 559. — 

 Cicatrisation de plaies cutanees en dehors de Forganisme. C. R. Soc. de Biol. 1911. 

 pag. 253. — Jolly, A propos des Communications de MM. Alexis Carrel et Montrose 

 T. Biirroivs sur la culture des tissus. C. R. Soc. de Biol. 1910. p. 470. — Observations 

 ä Foccasion de la communication de MM. Carrel et Burrows. C. R. Soc. de Biol. 

 1911. p. 4. — Lambert and Hanes, Growth in vitro of the transplantable sarcomas of rats 



