34 



Biological Chemistry. 



tion. The experiment should be commenced as rapidly as 

 possible after the animal has been killed, so as to avoid all 

 complications due to post-mortem changes. Various forms 

 of perfusion apparatus have been suggested. A compara- 

 tively simple one, due to Brodie, is described in this place. 

 The essential details are shown in the accompanying illus- 

 tration. It consists of a pump D which drives the blood 



J 



Fig. 25. 



into a glass receiver H. The tube K at the bottom of this 

 is connected by rubber or glass with a cannula tied into 

 the artery of the organ to be perfused. The cannula con- 

 nected with the vein, through which the fluid flows after 

 perfusion through the organ, is connected by rubber 

 tubing with the lateral inlet of the vessel A. This has 

 also another lateral tube 0, which is connected with the 

 inlet tube of the pump D, the outlet tube of which is con- 



