44 BACTERIA IN RELATION TO PLANT DISEASES. 



nodes. Examined these red bundles under the hand-lens, and thus far find no distinct evidence of 

 bacterial ooze. Most evidence of disease in this stem of any examined this morning, but it is not 

 seriously diseased. 



The numerous young shoots look so healthy that I will not cut them out. 



Old shoot. Bears label 926. Sound at base. Cane well matured. Sound on the surface. 

 Leafy toward the top and leaves healthy looking. Buds well matured. Cross-sections of the stem 

 from the top downward show the upper portion to be sound. At a distance of about 6 feet from the 

 ground stained vessels appear (3 or 4 in number) although the surface of the cane is sound in that 

 internode. Stain runs out in the next internode and appears in the third one below. Here again it is 

 confined to the middle of the stem and the middle of the internode, 3 or 4 bundles being reddened. 

 It appears again in the next internode, and runs out in the next below that. Appears again with a 

 greater number of vessels stained in the node below that. All the way down the exterior of the stem 

 is perfectly sound. The basal 2 feet of the stem appears to be free from stain. The cane is very 

 sweet and juicy. Examined these stained internodes under the hand-lens. I find no distinct evi- 

 dence of bacterial ooze ; will look at it under the microscope later. 



Old cane. Sound at the base. Basal buds well developed. Healthy looking, well-developed, 

 mature cane. Upper leaves green and healthy. On cross-section, find top perfectly healthy. About 

 6 feet from the ground I begin to get red-stained vessels in the middle of the stem (a few only) ; these 

 increase in number in the next internode, run out in the one below that, appear again in the next one, 

 are still more abundant in the one below that (always in the middle of the cane), run out in the one 

 below that, appear again in the next one, appear again in the next three, and disappear about 15 

 inches from the ground. The external portion of this part of the stem appears to be sound. The 

 interior of the basal portion of the stem is sound. Examined these red vessels under the hand-lens. 

 No immediate bacterial ooze. 



Old cane. Sound at the base. Good size. Sound externally. Leafy at the top only. Upper 

 leaves healthy looking. Cross-section of the upper part of the stem perfectly sound. Stem con- 

 tinues to be sound until one reaches a level of about 6 feet, when a few red bundles appear; these run 

 out a little lower down. Stem very tender, juicy and sweet. The red bundles appear again in the 

 third internode. They appear again in the next one sparingly, and in the next one below that ; I am 

 not sure that it may not be due to the age of the cane. The cane is fully ripe, if not over ripe. Basal 

 part of the cane sound. 



Old cane. Sound at base. Buds well developed. Stem sound externally. Leafy at the top 

 and leaves healthy. Only the lower 4 feet of the cane is naked. Cross-sections at the top show the 

 interior of the stem to be perfectly healthy. This state continues downward the whole length of the 

 cane. I believe this cane grew after the plants were inoculated. 



Old rather small cane. Sound at the base. Basal buds well developed. Stem sound exter- 

 nally. Upper part very leafy and perfectly healthy. Evidently grew after the inoculations. It 

 bears 17 healthy leaves. Cross-sections at the top perfectly sound. Whole interior of the cane 

 sound. 



Old cane. Bears labels 940 and 946. Sound at the base on cross-section and externally. Basal 

 buds well developed ; not pushing. Basal portion of stem naked. Only the upper part bears healthy 

 green leaves, the middle portion of the cane being covered with dead leaves. This cane is well devel- 

 oped and mature for a distance of more than 6 feet. On cross-section perfectly sound at the top and 

 for several feet, then a few red bundles begin to appear ; these run out in the next internode, appear 

 again in the second one, continue in the third and fourth one, are more abundant in the fifth one, and 

 still more in the sixth one. Much less in the seventh and eighth one, and run out in the ninth one at 

 a distance of 1 6 inches from the ground. The rest of the stem is free from internal stain until the fifth 

 short node from the ground, which also has a few red-stained bundles ; these run out in the next inter- 

 node under. Examined under the hand-lens : No distinct evidence of bacterial ooze. 



Old cane. Sound at the base. Basal buds well developed. Very leafy at the top, and leaves 

 healthy. I believe the plant grew after the inoculations were made. Cross-section at the top per- 

 fectly healthy; continues so for the whole length of the stem. 



Large old cane. Sound at the base. Leafy only near the top. Extreme upper leaves green. 

 Stem healthy within for a distance of many feet, that is, until one reaches a height of about 4 feet 

 from the ground, when some red bundles appear in several internodes. These continue until one 

 comes to about a foot from the ground, where they disappear. Basal part of the stem perfectly 

 healthy. Cane very tender, juicy, and sweet. Examined the red bundles under the hand-lens: No 

 distinct evidence of bacterial ooze. 



Old cane. Sound at the base. Basal buds well developed. Cane mature. Bears label 956. 

 Upper leaves well developed and green. On section of the stem upper part sound. About 5 feet 

 from the ground red stain begins in the middle of the stem and continues through several internodes, 



