STEWART'S DISEASE OP SWEET-CORN (MAIZE). 



141 



TABLE 19. Growth of Bacterium stewarti in Various Media. 



Each tub* was inoculated with > blf 3 mm. loop* of the cloudy fluid from * 6 d.y old peptonized beer-broth culture. (From notei of 



August 17, 1908.) 



Conclusion: Bactrrium stevarli will grow in this medium but has some difficulty in getting started. Most be inoculated copiously 

 tMedium evidently not entirely free from nitrogen. 



^Composed of: distilled water i.noo. dipotanium photphate 2.0: magnesium sulphate O.I; ammonium phosphate o.t; indium acrtate 5. o. 

 {September 21: Stock 5415 heavily clouded; stock 34*4. doubtful, if any. growth; stock 3*27. color ranch duller thai in the check, due 

 to clouding. 



Nitrogen foods. The behavior in Uschinsky's solution indicates ability to use sodium aspara- 

 ginate and ammonium lactate, one or both, under the conditions present. 



The inability to reduce potassium nitrate shows that nitrogen is not obtained from this source. 

 Peptone and the nitrogen in beef-juice are both foods. 



Tests made in 1908 with Bad. stewarti from three sources, inoculating copiously, gave the fol- 

 lowing results: 



(1) No growth or doubtful: Asparagin water (2 per cent asparagin); ammonium lactate and cane-sugar in water; 



ammonium citrate and cane-sugar in water; ammonium tartrate and cane-sugar in water. 



(2) Feeble clouding: Asparagin and cane-sugar in water. 



