1 62 BACTERIA IN RELATION TO PLANT DISEASES. 



NOTES ON CULTURAL CHARACTERS. 



Stabs in Cornmeal Agar (Stock 3794). 

 1909. 



Sept. 8. Inoculation from slant agar subculture of September 3, 1909. 

 Sept. 18. Stab growth fairly good to bottom of the tubes; finely saccate along line of stab. Surface growth pale yellow 



(almost white) ; largest diameter 4 mm. 

 Oct. 1 1 . Scanty, pale yellow surface growth. Stab growth moderate, better than in beef agar. 



Potato Cylinders (Stock 3856). 



Sept. 8. Inoculated from agar slant of September 3, 1909. 



Sept. 18. Growth moderate; slime canary yellow, thin, shining; potato slightly grayed, liquid somewhat yellowed. 



Oct. 1 1 . Growth moderate, spreading, thin, smooth, canary yellow, moderate yellow precipitate ; liquid clear not 



thickened; potato slightly browned. 

 Oct. 27. Some starch destroyed. Potato alkaline to litmus paper. 



Standard Nutrient Agar Stabs (Stock 3807). 



Sept. 3. Inoculation from colonies in plate of August 23, 1909, by Miss Bryan. 



Sept. 8. Surface growth i to 2 mm. in diameter; pale yellow; opaque. Slight saccate growth along line of stab. 



Sept. 18. Surface growth 10 mm. in diameter; canary yellow; smooth-shining, opaque; flat; viscid. Stab growth finely 



saccate. 

 Oct. 1 1. Same appearance as on September 18. The agar is drying and separating from the walls of the tubes. 



Nitrate Bouillon (Stock 3852). 



Sept. 8. Inoculation from agar slant of September 3, 1909. 



Sept. 18. Good growth: Tested for nitrites (used boiled starch water, potassium iodide, and sulphuric acid). No color 



reaction. Medium tested with diphenylamine gives dark blue reaction, showing presence of nitrate. 

 Sept. 27. Tested remaining cultures. No nitrites present. 



Cohn's Solution (Stock 3827). 



Sept. 8. Inoculated from agar slant of September 3, 1909. 



Sept. 1 8. No growth. 



Sept. 22. No growth. 



Oct. 1 1. No growth. Reinoculated from agar slant of September 29, 1909, 



Oct. 27. Cultures merely milky. Slight, white precipitate, readily dissolves. 



Cream-free Milk (Stock 3825). 



Sept. 8. Inoculated from agar slant of September 3, 1909. 



Sept. 1 8. Surface of milk is yellow (canary color) to a depth of 3 to 4 mm. ; also a rim of yellow 2 to 3 mm. wide. Milk 



in lower part of tube is cream color. No coagulation. 

 Sept. 22. Yellow surface layer now 4 to 6 mm. deep and translucent. 

 Oct. n. Surface layer now 10 to 12 mm. deep. Still yellow. The milk below is deep cream color, thick and smooth 



like butter. Some yellow precipitate in base. 

 Oct. 27. Yellow translucent whey, now 12 to 25 mm. deep. The curd is deep cream color. 



Litmus Milk (Stock 3775 lavender blue). 



Sept. 8. Inoculation from slant agar of September 3, 1909. 



Sept. 18. Wide (3to4mm.) rim and surface of canary yellow; the medium below this is uniformly pale gray (griseus 

 according to Sacc.). It is liquid throughout. 



Sept. 22. Color now quite near Ridgway's "drab" No. 13. Surface layer 4 to 6 mm. deep of a dull yellow trans- 

 lucent. 



Oct. 1 1. Yellow surface layer not broader than on th; 22d (Evaporation is more rapid than in plain milk). The color 

 is brighter yellow and transparent in parts. Below the yellow layer the milk is dirty cream color, thick- 

 ened but much less so than in plain milk cultures of same age. 



Oct. 27. Dirty cream-color curd in lower 12 to 15 mm. covered by 10 to 12 mm. of yellowish (tinge of orange-red at 

 top) translucent whey. Litmus reduced. 



Beef Bouillon (Stock 38$ i). 



Sept. 8. Inoculation from agar slant of September 3, 1909. 



Sept. 18. Moderate clouding; thin, white, flocculent masses suspended in medium. The moderate slimy precipitate 



rises in long strings; these break with shaking but do not readily dissolve. No rim or pellicle. 

 Oct. II. Moderate rolling clouds, clouding densest at surface. White patches of rim. No pellicle. Precipitate 



moderate; yellowish; viscid; rises in a swirl. 



Gelatin Stabs (Stock 3728). 



Sept. 8. Inoculation from slant agar of September 3, 1909. 



Oct. ii. Scant, canary yellow, smooth-shining surface growth. Either no trace of growth or slight in stab. No 

 liquefaction.* (Kept in refrigerator, temperature 14 to 15 C.) 



*In a later test started Oct. 29, 1912, liquefaction began Nov. 9; was stratiform and 4 to 7 mm. deep on Jan. 3, 

 1913, and complete June 27, 1913. Another set of tubes inoculated Jan. 8, 1913, were completely liquefied July 

 15, 1913. Both sets kept in ice-box at about i8"C. 



