254 BACTERIA IN RELATION TO PLANT DISEASES. 



19. Growth never followed inoculation into cultures with KNOs and glycocoll with levulose; with asparagin and 

 levulose only in4cultures from 2 sources. This is not strange, because also with asparagin alone growth occurred only 

 once. 



20. Development in all the cultures containing asparagin, glycocoll, and KNOs was extremely rare. Very often 

 only one or two of the three cultures showed growth when these were inoculated from the same culture of bouillon, of 

 KNO.i or of glycocoll. 



21. In general the number of cultures which succeeded with some of the alcohols was greater than with the cor- 

 responding sugars. 



22. In a great number of cultures visible growth was not synonymous with strong development. 



23. Because the Deli strains of Bacterium solanacearum are parasitic as well for Nicotiana tabacum and Capsicum 

 annuum (like Uyeda's B. nicotianae) as for egg-plant and tomato (like Uyeda's B. solanacearum Smith), and moreover 

 because nearly all of Uyeda's designated cultural differences fall away I think that B. nicotianae Uyeda is identical 

 with B. solanacearum Smith. 



(5) REPORT ON THE ATTEMPTS TO DISINFECT WITH CHEMICALS THE SEED-BEDS ON SLIME-SICK SOILS. 



On 20 plantations belonging to 1 1 companies tests were carried out, using potassium perman- 

 ganate, formaldehyd, and carbon bisulphide. The seedlings on 6 of the plantations were destroyed 

 by ants and crickets. Report is made of the results on each of the other 14 plantations which show 

 some success with the disinfectants, but not brilliant nor uniform. The entire subject is summed up 

 in the following conclusions : 



(1) That in spite of the disinfection of seed-bed and of the well-water used, when the seedlings 

 were planted on infected ground infections took place. 



(2) That this infection, as far as could be made out, took place more through the leaves than 

 through the roots, so that the infection must have been brought to them from without. 



(3) How this transmission took place (whether by animals or tools) must be followed up and 

 experiments are already under way. 



(4) It must also be looked into closer whether the disinfection of the whole seed-bed ground gives 

 an important advantage over the disinfection, simply, of the seed-beds, leaving the paths between 

 untreated. 



(5) That far away, in most cases, the plants were larger and more vigorous on the treated than 

 on the untreated seed-beds. 



(6) That in all cases where differences appeared after planting out, they were to the detriment of 

 the untreated seed-beds, so that it certainly is desirable to repeat the tests. 



(6) A CASE OF SLIME-SICKNESS IN DJATTI SEEDLINGS. 



This was found in Djatta plants [Tectona grandis] standing among diseased tobacco. The signs 

 appeared to be those of the slime-disease. Plate cultures were set and three sorts of bacteria were 

 isolated, one of which agreed culturally with B. solanacearum from slime-sick tobacco. Although the 

 identification by cultural tests left no room for doubt, inoculations were made into 4 tobacco seedlings, 

 all of which became diseased. Out of two of these plants the bacteria were again isolated and served 

 for new inoculations along with a strain isolated from tobacco. With these three strains 15 young 

 tobacco plants, on land not subject to the disease, were inoculated by a prick, 5 from each culture. 

 At the same time 9 young Djatti plants were inoculated, 3 from each culture. All of the tobacco 

 plants showed the disease at the end of a week ; the Djatti did not. Three days later the tobacco was 

 removed and the earth disinfected, although the disease was still confined to the top of the plant, i. e., 

 not yet in the roots. All the Djatti plants were now diseased, not only those inoculated from the 

 Djatti cultures, but also the 3 inoculated from the tobacco strain. No difference in virulence was 

 observed on Djatti plants, but on the tobacco DTi was much the more virulent. The checks were 

 sound, both the Djatti and the tobacco. 



With other cultures from the same source the inoculation experiment was repeated 4 days later on 

 a trial field on 15 Djatti seedlings with the surprising result that only one of them died, the others 

 being not much affected. Six weeks later, however, two other cases were found in the plants inocu- 

 lated with the Djatti bacteria. The disease was visible soon after a couple of showers and the bac- 

 teria were demonstrated microscopically in the stems more than 2 dm. above the point of inoculation. 



Later with DTi and T four tobacco plants in pots were inoculated, all of which became diseased 

 in 5 to 7 days. 



By inquiry Honing learned that on other plantations occasionally diseased Djatti had been 

 observed. 



(7) ON THE CONNECTION BETWEEN SLIME-SICKNESS IN SEEDLINGS AND IN PLANTED-OUT TOBACCO. 



In "Cause of the slime-sickness and attempts to combat it III," were cited some instances of 

 direct connection between disease in the seed-bed and in the field, even in cases where the disease in 

 the seed-beds was only sporadic and sometimes was not known. In "Report on the slime-disease 



