294 



INDEX. 



PAGB 

 Bacterium vascularum continued. 



inoculation experiments, Cobb's . 8, 12 



inoculation experiments, Tryon's J i 



inoculation, methods of 21, 27, 31, 33, 46 



inoculations, general remarks on 46 



inoculations on 



Blanca cane 34 



Caledonia Queen cane . . . . 33. 34. 4*> 



Cinta cane 34 



Common Green cane 21, 32. 34. 4 6 



Common Purple cane 



Crystalina cane 33. 34. 4 6 



Jamaica cane 33. 34. 4 6 



Louisiana cane, Variety No. 74 27, 34, 46 



Striped Green cane. . 31. 34, 46 



inoculations, pure-culture 11,21 



inoculations, variety tests 31, 33. 46 



inversion of cane-sugar 62 



isolation, methods of 54 



isolation from 



artificially infected sugar-cane 21, 24, 39, 46 



Australian cane 2 1 , 65 



red bundles ' 7. 5 ' 



red ooze 46 



yellow ooze 46 



lactose, effect on growth 64 



lesions produced 13 



levulose, effect on growth 64 



liquefaction of gelatin 57. 59. 67 



literature 71 



litmus, reaction 56, 57, 61, 67 



longevity, 



effect of temperature on 66 



in culture-media 65, 66 



in the host 65 



magnesium, effect on growth 64 



malic acid, effect on growth 63 



maltose, effect on growth 64 



maximum temperature '. . . 64 



measurements ' ' . , 54. 67 



milk. 



acids not formed from 61 



casein not precipitated 61, 67 



effect of organism on 6 1 , 62 



surface growth not conspicuous 61,62 



tyrosin not formed in 62 



with litmus becomes indigo blue 61 



morphology 7, 21, 54, 67 



motility, best seen in young cultures 54 



motility, often absent in old growths 54 



nitrates, not reduced by 61 



nitrogen foods 63, 67 



one-flagellate 3. 2 1 , 55, 56 



optimum temperature 65 



oxalic acid, effect on growth 63 



pathogenicity 8, 2 1 



phenolphthalein, reaction of organism to 61 



phosphates, effect on growth 57. 64 



potassium, effect on growth 64 



potassium nitrate as nitrogen food 63 



potato, growth on 60, 65 



poured-plate cultures, description 54 



pseudozoogloeae 54, 61 



pure-cultures from diseased cane 21 



red bundles, isolation from . . .,.,... 17, 51 



reddening of bundles 16 



(See also Sugar-cane, Cobb's disease of.) 



reduction of nitrates 61 



relation to B. sacchari Spegazzini 88 



resume of salient characters 66 



resuscitation from old diseased canes 46 



saccharose, action on 62 



saccharose, as carbon food 63 



saccharose, effect on growth . 64 



salts, effect on growth 64 



PAGS. 

 Bacterium vascularum continued. 



slime, oozing from leaf-sheaths and stems 16, 22,25,53,66 



Smith, R. Greig, studies 57, 60, 61, 62, 63, 64 



(See also Sugar-cane, Cobb's disease of.) 



sodium chloride, effect on growth 63, 64 



spores 54 



stains, reaction to 54 



starch, effect on growth 64 



starch, slight action of organism on 60 



substitution for B. pseudarabinus in Greig-Smith's 



experiments on red stain in gummed cane ... 50 



sugars as food 63, 64 



sunlight, organism sensitive to 66 



tear-drop formation on gelatin 59. 67 



temperature, effect on longevity 66 



temperature relations 64, 67 



thermal death-point experiments 64, 65 



tissues attacked 6, 7, 15, 24, 66 



trimethylamin, production of 56 



Tryon's studies 1 1 



(See also Sugar-cane, Cobb's disease of) 



Uschinsky's solution, feeble growth in 62 



vascular system attacked by 6, 15, 24, 66 



vasculin 7 



viscidity 56, 61, 66 



vitality in stem and on media 65, 66 



yellow slime, 



primrose to lemon color on potato 60 



whitish in absence of air 58 



Bacterium xanthochlorum, 



(See also Potato, Schuster's German Rot.) 



acid, toleration of 275 



acid-production 272, 275 



aerobism 273 



alcohols, growth in 273, 275 



alkali, toleration of 275 



alkali-production 272, 275 



ammonia-production 272 



amylase produced 272 



anaerobism, facultative 273 



biological characteristics 272 



blood-serum, non-liquefaction of 273 



chloroform, effect of 275 



colonies on agar 273 



colonies on gelatin 273 



disintegration of potato-tuber sections in infected 



potato-juice 274 



drying, sensitive to 275 



entrance of organism into tuber 272 



enzym, action on starch 274 



effect on cell-walls 274 



effect of temperature on 274 



extraction of 274 



injection into potato-tubers 274 



fermentation-tube experiments 275 



filaments, production of . . 272 



flagella 272 



effect of temperature on formation of 272 



fluorescence, greenish-yellow. 273 



galactose, fermentation of 275 



gas, non-production 273, 275 



gelatin, liquefaction 273, 274 



glycogen, production of 272 



Gram's stain 272 



grape-sugar, fermentation of 275 



growth on, or in, 



acid bouillon 275 



agar (plus 15) 275 



alkaline agar 273 



alkaline bouillons 275 



alkaline potato-agar 274 



alkaline potato-agar plus tyrosin 274 



beer 273 



bouillon over chloroform 275 



bouillon plus grape-sugar 273 



bouillon, pcptonized, plus sodium chlorid 275 



