CONTENTS. 



OUTLINE OF METHODS OF WORK. 



Page. 



General Remarks 3 



The Disease 4 



Previous Literature 6 



Geographical Distribution 7 



Signs of Disease 7 



Pathological Histology 8 



Direct-infection Experiments 9 



The Organism 9 



Pathogenesis 9 



Rules of Proof 9 



Morphology 18 



Size, Shape, etc 18 



Capsules 19 



Flagella 20 



Spores Endospores, Arthrospores 21 



Cell-unions Zoogloeae, Chains, Filaments. . 22 



Involution-forms 23 



General Comment 23 



Physiology 25 



Motility 26 



Growth 27 



Chemotropism 27 



Reaction to Stains 27 



Culture-media 29 



Nutrient Gelatin 29 



Nutrient Agar 31 



Silicate-Jelly 36 



Solid Vegetable Substances 39 



Plant Juices (with and without the addi- 

 tion of water) , 41 



Animal Fluids 45 



Beef-broth 45 



Milk 46 



Litmus Milk 48 



Rice cooked in Milk 48 



Loeffler's Solidified Blood-serum 48 



Egg-albumen 48 



Egg-yolk 49 



-Synthetic Media and Other Special Media. 49 



Relation to Free Oxygen 51 



Surface and Deep Growths 51 



Fermentation-tubes 5 2 



Growth in Hydrogen, in Carbon Dioxide, 



in Vacuo, and in Nitrogen 54 



Luminosity 60 



The Organism Continued. 

 Physiology Continued. 



Fermentation-products 60 



Alkalies (Ammonia, Amins, etc.) 61 



Reducing Powers 62 



Hydrogen Sulphide 62 



Mercaptan and Other Odors 62 



Indol, Phenol, Leucin, Tyrosin, etc 62 



Reduction of Nitrates, etc 63 



Fixation of Free Nitrogen, etc 64 



Assimilation of Carbon Dioxide 64 



Pigments 64 



Crystals 66 



Enzymes 66 



Sensitiveness to Plant Acids 69 



Sensitiveness to Alkalies 69 



Effect of Desiccation 70 



Effect of Direct Sunlight 71 



Vitality on Various Media 72 



Mixed Cultures and Mixed Infections 72 



Reaction to Antiseptics and Germicides 74 



Thermal Relations: Maximum, Minimum, 

 and Optimum Temperatures for 

 Growth; Thermal Death-point; Effect 



of Freezing 75 



Other Host-plants 87 



Pathogenic or Non-pathogenic to Animals . . 88 



Economic Aspects. 90 



Losses 90 



Natural Methods of Infection 91 



Conditions favoring Spread of the Disease. ... 93 



Methods of Prevention 93 



General Considerations 94 



Location of the Laboratory 94 



Equipment of the Laboratory 94 



Care of the Laboratory 96 



Preparation and Care of Culture-media 97 



The Cleaning and Sterilization of Glassware 



and Instruments 100 



Making and Transference of Pure Cultures. . . 103 



The Final Disposal of Infectious Material 106 



Methods of Inoculation 108 



The Keeping of Records 109 



The Making of Collections 117 



Distilled Water 124 



