FORMULA. 



I 97 



SYNTHETIC CULTURE MEDIA. 



Pasteur's Culture Fluid. 



Ammonium tartrate 10 



Ashes of yeast 10 



Rock candy 100 



Distilled water 1,000 



Dissolve cold. 



Naegeli's Nutrient Solution. 



Calcium chloride o.i 



Magnesium sulphate .2 



Dipotassium phosphate i.o 



Ammonium tartrate 10.0 



Distilled water 1,000.0 



Cohn's Nutrient Solution. 



Distilled water 1,000.0 



Acid potassium phosphate 5.0 



Magnesium sulphate 5.0 



Neutral ammonium tartrate 10.0 



Potassium chloride 0.5 



(De Bary, p. 86, Vorles. ii. Bact., 2 Auflage.) 

 Raulin's Culture-Fluid. 



Distilled water 1,500.00 



Granulated cane sugar 70.00 



Tartaric acid 4.00 



Ammonium nitrate 4.00 



Ammonium phosphate .60 



Potassium carbonate .60 



Magnesium carbonate .40 



Ammonium sulphate .25 



Zinc sulphate .07 



Ferrous sulphate .07 



Potassium silicate .07 



Prasmowski's Culture-Fluid. 



Dipotassium phosphate 5.0 



Magnesium sulphate 5.0 



Ammonium carbonate 5.0 



Calcium chloride .5 



Distilled water 1,000.0 



Dissolve cold. Any desired sugar may be 

 added for the carbon food. 



Adolf Mayer's Culture-Fluid. 

 (Unters. ii. d. ale. Gahr., 1870.) 



Magnesium sulphate 10.0 



Ammonium nitrate 15.0 



Tri-basic calcium phosphate .1 



Potassium phosphate IO.O 



Distilled water 1,000.0 



Dissolve cold and add sugar. Add sodium 

 chloride (3 per cent) if it is to be used for 

 luminous bacteria, and an excess of pure car- 

 bonate of lime if acid-forming bacteria are to be 

 grown. 



Uschinsky's Solution. 



Distilled water 1,000 



Glycerin 30 to 40 



Sodium chloride 5 to 7 



Calcium chloride o.i 



Magnesium sulphate 0.3 to 0.4 



Dipotassium phosphate 2 to 2.5 



Ammonium lactate 6 to 7 



Sodium asparaginate 3tO4 



Modified Uschinsky's Solution. 

 The modified Uschinsky recommended by the 

 writer for use with starch jelly is made as fol- 

 lows: 



Distilled water 1,000.000 



Ammonium lactate 5.000 



Sodium asparaginate 2.500 



Sodium sulphate 2.500 



Sodium chloride 2.500 



Dipotassium phosphate 2.500 



Calcium chloride .010 



Magnesium sulphate .010 



Fraenkel and Voges' Solution. 

 (Hygienische Rundschau, Bd. IV, 1894, p. 769.) 



Water 1,000 



Sodium chloride 5 



Dipotassium phosphate* 2 



Ammonium lactate 6 



Sodium asparaginate 4 



This paper also discusses Uschinsky's solution. 



Fermi's Culture-Fluid. 



Distilled water 1,000.0 



Magnesium sulphate .2 



Acid potassium phosphate i.o 



Ammonium phosphate 10.0 



Glycerin 45.0 



This may be added to agar in place of pepton- 

 ized beef-broth (De Schweinitz) or to silicate 

 jelly, in which case the volume of water must 

 be reduced (see Silicate Jelly, p. 36). 



Moore's Culture-Medium for Leguminous Root- 

 tubercle Bacilli. 

 (For field use.) 



The dried culture (on cotton) is thrown into 

 clean water containing : Cane-sugar, I ; c. p. 

 monopotassium phosphate, o.i; c. p. magnesium 

 sulphate, o.oi per cent. After 24 hours add 

 Merck's pure dibasic ammonium phosphate to 

 amount of 0.5 per cent. Seeds are drenched 

 with this fluid at end of another day, dried in 

 shade, and planted. 



* l.i-i mi ami ami Neumann recommend neutral commercial sodium phosphate ('. c. p. 29.) 



