SYNTHETIC CULTURE MEDIA. 



2OI 



Ortol Developer. 

 (See pp. 140-141.) 



Pyro Developer for Dry Plates. 

 (Recommended by S. G. Lofft.) 



(1) Water iooz., or 300 cc. 



Citric acid 10 grains, or 6.46 grams 



Pyrogallic acid... I oz., or 31 grams 



(2) Sodium sulphite 



(crystals) 402., or 124 grams 



Water 16 oz., or 480 cc. 



Or 



Seed's sulphite. ...i 5^ oz., or 46.5 grams 

 Water i6oz., or 480 cc. 



(3) Sodium carbonate 



(crystals) 4oz., or 124 grams 



Water i6oz., or 480 cc. 



Or 

 Seed's carbonate.. 2oz., or 62 grams 



Water i6oz., or 480 cc. 



To develop take 



Water 4 oz., or 120 cc. 



No. i 2 drams, or 7.5 cc. 



No. 2 J'a oz., or 15 cc. 



No. 3 1 A oz., or 15 cc. 



For underexposures dilute and change fre- 

 quently to fresh developer. 



For overexposures use old developer or re- 

 strain with a few drops of 10 per cent potassium 

 bromide. 



Lantern-slide Developer. 



(Used in Laboratory of Plant Pathology.) 



Distilled water cc. . 900 



Carbonate of soda (cryst.) grams. . 39 

 Sulphite of soda (cryst.). ..grams. . 39 



Hydrochinon grams.. 13 



Add the hydrochinon after solution of the 

 soda salts, and put at once into a glass-stoppered 

 bottle. For use take 3 ounces of above and 3 

 ounces of distilled water, to which add 5 drops 

 of a 10 per cent solution of bromide of potas- 

 sium. If properly exposed the image should 

 appear in 30 to 60 seconds, and the development 

 should be over in 3 or 4 minutes. Good for a 

 dozen or more properly exposed slides. 

 Zettnow's Copper-chrom-filter. 



Dry, pure, copper nitrate 160 



Pure chromic acid 14 



Distilled water 250 



This may be diluted further with water if 

 desired. 



In case there is difficulty in preparing the 

 above, a solution, stated by Zettnow to be nearly 

 as useful, consists of 



Sulphate of copper 175 



Bichromate of potash 17 



Water 1,000 



(Centralb. f. Bakt., IV Bd., 1888, p. 51.) 

 Neuhaus says, water 500 to 1,000, and also 

 2 cubic centimeters of sulphuric acid. This so- 

 lution extinguishes all the blue and violet rays. 



Toning Bath for Solio Paper. 



(A) Hyposulphite of soda. .. 8 ounces 

 Potash alum (crystals).. 6 ounces 



Cane sugar 2 ounces 



Water 80 ounces 



Dissolve cold, then add 2 ounces of borax 

 which has been dissolved in 8 oz. of hot water. 

 Let stand 12 hours, and then decant. 



(B) Pure chloride of gold. . 7.5 grains 



Acetate of lead 64 grains 



Distilled water 8 ounces 



This solution must not be filtered and must 

 be shaken thoroughly each time before using. 

 Solio paper should be printed about one-third 

 darker than it is desired to have the pictures. 

 When the prints are ready they are placed face 

 down in a toning mixture made of stock A, 8 

 ounces, and stock B, i ounce, taking care that 

 the entire surface of each print is uniformly 

 wetted. They are allowed to remain in this 

 bath, with constant movement by means of the 

 fingers, until the desired color is obtained (usu- 

 ally about 5 minutes). The prints are now im- 

 mersed in salt water (i -.32) for 5 minutes. 

 They are then exposed for 15 minutes to the 

 fixing bath, consisting of 



Hyposulphite of soda I ounce 



Sulphite of soda (crystals) 60 grains 



Borax 1 A ounce 



Water 20 ounces 



The prints are finally washed for from i to 2 

 hours in running cold water. The toning bath 

 should not be cooler than 40 or warmer than 

 60 F. 



A New Test for Indol. 



Herter & Foster have recently described what 

 is stated to be a rapid and accurate method of 

 determining indol, adapted either for colorimet- 

 ric or gravimetric determinations. To slightly 

 alkaline solutions naphthoquinon sodium mono- 

 sulfonate is added. A blue crystalline com- 

 pound, di-indyl naphtho-ketone mono-sulfonate 

 is produced. This is slightly soluble in water, 

 but is readily soluble in chloroform, its solution 

 being red. (Science, n. s., Vol. XXI, 1905, 

 p. 987.) 



