CAPSULES; STAINS AND STAINING METHODS. 



221 



('92). LIESENBERG, C., u. ZOPF, W. Ueber den sogen- 

 annten Froschlaichpilz (Leuconostoc) der 

 europaischen Ritbenzucker- und der javan- 

 ischen Rohrzuckerfabriken. Zopf s Beitraege, 

 Hft. I, 1892, pp. 1-29, with 2 plates, and 

 Hft. ii, 1892, pp. 1-2. Rev. in Am. Nat., 

 March, 1897, p. 228. 



('92). WELCH, WM. H. A gas-producing bacillus 

 (B. aerogenes capsulatus, nov. spec.) capable 

 of rapid development -in the blood-vessels 

 after death. Bull. Johns Hopkins Hospital, 

 vol. 3, No. 24, July-August, 1892, pp. 81-91. 

 Also a separate. 



('94). KOCH, ALFRED, AND HOSAEUS, HANS. Ueber 

 einen neuen Froschlaich der Zuckerfabriken. 

 Centralb. f. Bakt, Bd. xvi, 1894, pp. 225- 

 228, mit i fig. 



Describes a branching gelatinous organism as Bact. 

 pedlculatum. The branching is due to a terminal split- 

 ting or one-sided development of the capsule. 



('95). STIFT, A. Ueber die in den Produkten der 

 Zuckerfabrikation auftretenden Bakterien. 

 Centralb. f. Bakt., 2 Abt., Bd. i, 1895, pp. 

 277-283. 



('96). WILDE, MAX. Ueber den Bacillus pneumonia: 

 Friedlander's und verwandte Bakterien. 

 Inaugural Dissertation, Bonn, 1896, pp. 74. 

 Bibliography of 89 titles. Printed by Carl 

 Georgi. 



('96). FRICKE, CARL. Ueber den sogenannten Bacil- 

 lus mucosus capsulatus. Zeitschr. f. Hyg., 

 Bd. xxm, 1896, pp. 380-451, with a bibliog- 

 raphy of 25 titles. 



('98). PANE, N. Ueber die Genesis der Kapseln des 

 Pneumococcus. Centralb. f. Bakt., xxiv Bd., 



1898, pp. 289-294, with 2 1 figs. 



The capsule is the swollen outer part of the bacterium. 

 ('98). KAUFMANN. Eine neue Methode zur Farbung 

 von Bakterienkapseln. Hyg. Rundschau, 

 1808, No. 18. Abstr. Centralb. f. Bakt., i 

 Abt., Bd. xxv, .p. 32. 

 ('99). WARD. See XLVII. 

 ('99). MOORE, A. Capsule staining. Trans. Jenner 



Inst., 2d ser., pp. 244, 1899. 



Recommends a contrast stain for the capsule. The 

 preparation is first fixed with dilute acetic acid, then 

 stained in carbol fnchsin for about i minute, washed in 

 distilled water and dried. It is then stained, with or 

 without gentle heat, in night blue for I or 2 minutes, 

 washed and dried. The night blue solution is Morton's 

 modification of McCrorie's stain. (See Morton, Flagella 

 staining.) Hy this method the capsule is stained blue and 

 the body dark red. 



('99). STRONG, LAWRENCE WATSON. Ueber die Kap- 

 selbacillen. Centralb. f. Bakt., xxv Bd., 



1899, pp. 49-52. 



('oo). BONI, ICILIO. Methode zur Darstellung einer 

 " Kapsel " bei alien Bakterienarten. Centralb. 

 f. Bakt., xxviii Bd., 1900, pp. 705-707. 



Coi). WARD. See XLVII. 



('oi). BONI, I. Ricerche sulla capsula dei batteri. 

 Giorn. Soc. ital. igiene, Milano, vol xxm, 

 1901, pp. 417-430. 



('02). SMITH, R. GREIG. An Ascobacterium from 

 the sugar-cane, with notes upon the nature 

 of the slime (Bacterium saoohari, n. sp.). 

 Proceedings of Linnean Soc. of New South 

 Wales, vol. xxvn, 1902, part i, pp. 137-145. 

 i plate. Also a separate (issued Aug. 22, 

 1902). See also Centralb. f. Bakt., 2 Abt, 

 Bd. ix, p. 806. 



XIV. Stains and Staining Methods. 



(Sec also XI, XII, and XIII.) 



(' ) EHRLICH. Beitrage zur Kenntniss der Anilin- 

 farbungen und ihrer Verwendung in der 

 mikroskopischen Technik. Arch. f. mikr. 

 Anat. Bd. xm, p. 263. 



('75). WEIGERT. Farbung von Bakterien. Ber. tiber 

 d. Sitzungen d. schlesischen Gesellsch. f. 

 vatenl. Cultur, 10 Dec., 1875 

 ('77). KOCH. See LV. 



('81). WEIGERT. Zur teohnik der mikroskop. Bak- 

 terienuntersuchungen. Virch. Archiv. Bd. 

 LXXXIV, 1881, p. 275. 



('82). EHRLICH. FaPbung der Tuberkelbacillen 

 Verhdlgn. d. Ver. f. i. Med., i Mai, 1882 

 D. med. Woch., 1882, p. 269. 



('82). ZIEHL. Zur Farbung des Tuberkelbacillus. 

 Deutsche med. Wochenschr., 1882, No. 33, 

 P- 451. 



('82). SZYSZYLOWICZ, J. Das Corallin als mikro- 

 chemisches Reagens in d. Pflanzenhistologie, 

 R. i. S. Ak. Krakau, T. x, 1883, pp. 97-114. 

 Not seen. Rev. Bot. Centralb., 1886, Bd. 

 xxvm, p. 51. See also Bot. Centralb., Bd. 

 xn, 1882, p. 139. 



This substance should be dissolved in sodium carbon- 

 ate. It is used as a test for slime derived from starch 

 which stains diffusely purple or rose. After staining the 

 preparation should be subjected to hot alcohol, which 

 removes the stain from all but the starch grains and 

 shine derived therefrom. Gum slime and cellulose slime 

 are bleached, the former even in cold alcohol. Pure gum 

 does not stain . Mount the preparation in Canada balsam. 



('83). ZIEHI,. Zur Lehre von den Tuberkelbacillen, 

 insbesondere iiber deren Bedeutung fiir 

 Diagnose und Prognose. Deutsche med. 

 Woohenschr., 1883, p. 62 



('84). KOCH. See vi. 



('84). LOEFFLER. See vi. 



('84). GRAM, C Ueber die isolirte Farbung der 

 Schizomyceten in Schnitt-und Trocken- 

 praparaten. Fortsohr. d. Med., Bd n No 

 6, 1884, pp. 185-189. 



('86). PFEFFER, W. Ueber Aufnahme von Anilin- 

 far.ben in lebende Zellen. Untersuchungen 

 a. d. bot. Inst. Tubingen, n, p. 179, 1886. 



('87). UNNA. Die Rosaniline und Pararosaniline, 

 eine bacteriologische Farbenstudie. Derma- 

 tologischen Studien. Heft 4, pp. 9-73. 

 Hamburg, Voss, 1887. 



('87). KUEHNE. Ueber ein combinirtes Universal- 

 verfahren, Spaltpilze im thierischen Gewebe 

 naohzuweisen. Dermatol. Studien, heraus- 

 geg. von Unna. Heft 6. Hamburg, L. Voss, 

 1887. 



('87). HEIM, L. Die Neuerungen auf dem Gebiete 

 der bakteriologischen Untersuchungsmetli- 

 oden seit dem Jahre, 1887, I. Farbungs- 

 methoden. Centralb. f. Bakt., x Bd., 1891, 

 pp. 260-265, 288-296, and 323-328. 

 ('88). KUEHNE, H. Praktische Anleitung zum mikro- 

 skopischen Naohweis der Bakterien im 

 thierisohen Gewebe. Leipzig (Giinthers), 

 1888, pp. 15-23, Methylenblaumethode. 

 ('88). UNNA, P. G. Die Entwicklung der Bak- 

 terienfarbung. Eine historisch-kritische 

 Uebersioht. Centralb. f. Bakt., 1888, in Bd., 

 pp. 22-26; also pp. 61-63, 93-99, 120-125, 153- 

 158, 189-195, 218-221, 254-259, 285-291, 312- 

 . 320, 345-348. 

 At the end 71 papers on this subject are mentioned by 



