METHODS OF WORK; MEANS OF DIFFERENTIATING. 



229 



fo2). CARNOT, PAUL, ET GARNIER, MARCEL. Sur la 

 technique des cultures en tubes de sable. 

 Paris, C. R. soc. biol, T. LIV, 1902, pp 748- 

 750. 



('02). CARNOT, PAUL, ET GARNIER, MARCEL. De 

 remploi des tubes de sable comme methode 

 generale d'etude, d'isolement et de selection 

 des microorganismes mobiles. Paris, C. R. 

 soc. biol, T. LIV, 1902, pp. 860-863. 



('02). REGAUD, CL. Nouveau bain de paraffine a 

 chauffage et regulation electriques. J. anat. 

 physiol, Paris, T. xxxvm, 1902, pp. 193- 

 214, av. fig. 



('02). GRIJNS, G. Eine einfache Vorrichtung, um 

 zu verhindern, dass beim Gebrauch des 

 Briitapparates fur konstante niedrige Tem- 

 peratur, System Lautenschlager (Katalog 

 No. 60, No. 117), wenn das Eis im Behalter 

 ausgeht, das ungekiihlte Wasser in den 

 kalten Sohrank fliesst. Centralb. f. Bakt, 

 Abt. i, Bd. xxxi, Originale, 1902, pp. 430-432. 



('02). HARRIS, NORMAN MACLEOD. Concerning an 

 improved method of making collodium sacs. 

 Centralb. f. Bakt, Abt. I, Bd. xxxn, Orig- 

 inale, 1902, pp. 74-80. 

 Dr. Harris makes his sacs around gelatin capsules. 



(03). GORSLINE, CHARLES S. On the preparation 

 and use of collodium sacs. Vaughan Quarter 

 Century Book, 1903, pp. 390-394. Ann Arbor, 

 Mich, George Walir. 



('03). WINSLOW, C. E. A, AND NIBECKER, C. P. The 

 significance of bacteriological .methods in 

 sanitary water analysis. Technology Quar- 

 terly, vol. xvi, 1903, pp. 227-239. Also a 

 separate. 



('04). REMLINCER, P. Le passage du virus rabique 



a travers les filtres. 2e mem. Ann. de 1'Inst. 



Pasteur, T. xvm, 1904, pp. 150-164. 

 ("04). Report of the [English] committee appointed 



to consider the standardisation of methods 



for the bacterioscopic examination of water. 



Journal of State Medicine, August, 1904. 



See also Chemical News, vol. xc, Oct. 7, 



1904, pp. 177-179. 



Distilled-water agar and distilled-water gelatin are 

 recommended for use along with nutrient agar and 

 gelatin. In the search for B. coli the committee recom- 

 mends either the glucose-formate broth of Pakes or the 

 bile-salt broth of MacConkey. 



XVIII. Special Means of Differentiating Bacteria. 

 ('84). GRAM. See xiv. 



('87). VON ROZSAHEGYI, A. Ueber das Zuchten von 

 Bakterien in gefarhter Nahrgelatine. Cen- 

 tralb. f. Bakt., ii Bd., 1887, No. 14, pp. 418-424. 

 Author added various substances to his culture media 

 e. g. fuchsin, methylen blue, gentian violet, vesuvin, etc. 

 Different bacteria behave very differently as respects 

 growth, absorption of pigment, and change of color in 

 the pigment. Organisms may l>e differentiated in this 

 way. 



('88). BUJVVID, O. Neue Methode znm Diagnos- 

 ticiren nnd Isoliren der Choierabakterien 

 Centralb. f. Bakt., 1888, iv Bd., pp. 494-496. 



11124 hours, in 2 per cent feebly alkaline peptone solu- 

 tion at 37 C., the cholera organism gives a fine purple-red 

 color on adding HC1. On longer cultivation other organ- 

 isms give the same reaction. This depends on the forma- 

 tion of indol and a trace of nitrite. 



('90). PETRUSCHKY, JOHANNES. Die Farbenreaktion 

 bakterieller Stoffwechselprodukte auf Lack- 

 mus als Beitrag zur Charakteristik und als 

 Mittel zur Unterscheidung von Bakterien- 

 arten. Centralb. f. Bakt., vn Bd., 1890, pp. 

 1-8 and 49-53. 



('92). Bujwip, ODO. Eine neue biologische Reaktion 

 fur die Choierabakterien. CentraJb. f Bakt 

 xn Bd, 1892, pp. 59S-S96. 



('92). BEYERINCK, M. W. Notiz fiber die Cholera- 

 rothreaktion. Centralb. f. Bakt, xn Bd 

 1892, pp. 715-718. 



('93). SCHILD. Formalin zur Diagnose des Typhus- 

 bacillus. Centralb. f. Bakt, Bd. xiv, 1893, 

 pp. 717-718. 



The typhoid organism will not grow in bouillon con- 

 taining as little formalin as 1:15,000. B. coli develops 

 vigorously with as much formalin as 1:3000. 



('93). MATHEWS, ALBERT P. On Wurtz's Method 

 for the Differentiation of Bacillus typhi 

 abdominalis from Bacillus coli communis, 

 and its application to the examination of 

 contaminated drinking water. Technology 

 Quarterly, vol. vi, 1893, pp. 241-251. 



Litmus lactose gelatin or agar is reddened by B. coli 

 and is unchanged or made deeper blue by B. typhosus. 



('93). GORINI, KONSTANTIN. Anmerkung iiber die 

 Cholerarotreaktion. Centralb. f. Bakt, Bd 

 xni, 1893, pp. 790-792. 



A good peptone for this purpose must be white, without 

 odor, entirely soluble in water, especially on warming 

 The water solution must be clear, colorless, neutral or 

 slightly alkaline, foamy on shaking. It must give a vio- 

 let reaction with Fehling's solution, which does not 

 change on boiling. It must give no nitrite reaction with 

 Gnesz reagent, and finally must give, after about 5 min- 

 utes with Diphenylamine, a faint but distinct narrow 

 clear blue ring. 



('94)- SCHNEIDER, PAUL. Die Bedeutung der Bak- 

 terien farbstoffe fiir die Unterscheidung der 

 Arten. (Inaug. Diss.) 8vo, 46 pp, 2 Taf, 

 Basel, 1894. Rev. in Centralb. f. Bakt, Bd 

 xvi, 1894, p. 633. See also Arbeiten a. d. 

 bakt. Institut Karlsruhe I, 1894. 



('94)- MARPMANN. Zur Unterscheidung des Bacillus 

 typhi abdominalis vom Bacillus coli com- 

 mune. Centralb. 1 Bakt, Bd. xvi, 1894, pp. 

 817-820. 



the first two. 



('94). LUNKEWICZ, M. Eine Farbenreaktion auf die 

 salpetrige Saure der Kulturen der Cholera- 

 bacillen und einiger anderer Bakterien 

 Centralb. f. Bakt, Bd. xvi, 1894, pp. 945-949. 



Describes the preparations of the Griess-Ilosvav re- 

 agent. 



('94). ABEL, RUDOLF. Ueber die Brauchbarkeit der 

 von Schild angegebenen Formalinprobe zur 

 Differential-Diagnose des Typhus bacillus 

 Centralb. f. Bakt, Bd. xvi, 1894, PP. 1,041- 

 1,046. 



Finds some bacteria more tolerant of formalin than 

 was stated by Schild. It is not a satisfactory method lor 

 separating typhoid bacilli from B. coli 



