1 84 



BACTERIA IN RELATION TO PLANT DISEASES. 



Of the inoculated trout one died at the end of 15 days. The rest (with one exception) 

 died in from 30 to 40 days from the time of inoculation. The relative rate of death of 

 inoculated and check trout is shown in the following table : 



Inoculated Trout. 



TABLE SHOWING DATE off DEATH AND SYMPTOMS OBSERVED IN TROUT. 



Check Trout. 



Date. 



1908. 

 Apr. 4 

 Apr. 22 

 Apr. 23 



Apr. 25 

 Apr. 28 

 Apr. 29 



Apr. 30 

 May i 

 May 2 

 May 4 



May 5 



May 7 

 May li 



May 19 



No. 



VI. I 



II. i 



VI, I 



I, i 



XIV, i 



II, i 

 IV, I 



V, i 

 IV, i 



XIV, i 



IV, i 



III, I 

 I, I 



XIII, i 



V, i 



III, I 



XIII, I 



XIV 



Remarks. 



Remarks. 



Congestion in region of anus, base of tongue, liver, heart. 



Eye-socket inflamed. Liver diseased. 



Hard yellowish rough tumor on tongue (the only hard tumor). Eye- 

 socket inflamed. 



Walls of lower intestine inflamed, ulcers on inner belly wall and 

 externally at root of pectoral fin and on outside below anal fin. 

 Throat sound. 



Amemic. Ulcerous tongue and inner belly wall. 



Anaemic. Eye-sockets inflamed. Vicinity of thyroid inflamed and 

 slightly swollen. 



Eye-socket inflamed. Liver white-mottled. Spots on gills and 

 region of the thyroid inflamed. 



Stomach and intestines much inflamed. White patch on liver. 

 Back part of throat inflamed, i. e., below the tongue. Inflammation 

 in throat where needle-pricks ended; None outside. 



Marked inflammation of abdominal wall where needle entered. Needle 

 wound healed externally. Inflamed spot at base of tongue. Lower 

 intestine much inflamed. 



Bloody patches on lower intestine. Ulcerontail. 



Several small ulcers on surface. Throat sound, liver diseased, in- 

 flamed patches on inner wall of abdomen. 



Small abrasion on surface, also a Saprolegnia patch (3 sq. in.). Gills, 

 stomach and intestine congested. Eye-socket badly inflamed. 



Eye on inoculated side is white-clouded, swollen inflamed tissue at 

 base of eyes, especially on inoculated side. Marked congestion of 

 the inner wall of abdomen in lower part; tongue somewhat swollen 

 and red. 



Small sore spot at roots of the tail. Throat sound. Membrane cover- 

 ing the intestine and eye-socket highly inflamed. Also inflamed 

 places on the inner lower belly wall. 



No tumor on the adipose (where inoculated) . Base of tongue swollen 

 and inflamed outside and inside. 



Throat and gills sound, eye-socket inflamed, viscera swollen and 

 inflamed. Inner belly wall badly inflamed. 



One alive on this date when experiment was abandoned. 



From Mar. 20 to Apr. 29. no deaths among 



the loo check fish. 

 During this period 10 deaths among the 18 



inoculated fish. 

 On Mar. 25, 3 showing throat tumors were 



separated from the rest of the checks. 



Apr. 30 i . The first check to die 

 in throat. 



Tumor 



May 6. Two, with cancerous growths in 

 throat. 



May 8. i. Cancerous thyroid. 



May 9. i . Mouth sore. 



May n. 7. Two have sores in the mouth. 



May 14. 3. One ansmic and with an ulcer 



on the gills. Weather hot for last three 

 days. 



May 15. i. Cancer in thyroid region. 



May 1 6. 4. One has a cancerous throat. 



May 19. 7. Not dissected. 



Since the above paragraphs were written the most hopeful portions of the inoculated 

 trout have been infiltrated, sectioned, stained, and studied, with the following results 

 (the figures in parenthesis refer to the preceding table). 



519 (April 28, V). Ulcer on inner wall of abdomen. Proliferations too regular for sarcoma. 

 Probably not malignant. 



520 (April 25, XIV). Hypodermic. Ulceron inner belly wall near en trance of needle. Sarcoma (?). 

 Very suspicious. 



546 (April 23, IV). Proliferations not malignant. 

 549 (April 25, II). Tumor on tongue : Adenocarcinoma. 



621 a (April 23, I). Portion of tongue. Probably not malignant. Some of the cartilage has 

 an abnormally large number of cells in it (chondroma?) . 



622 (April 12, II). Inflamed part of inner wall of abdomen. Proliferations but nothing 

 definite. 



642 (April 19, XIV). Sections of small swellings on inner belly wall where inoculated. May 

 be accounted for as simple inflammation. Later: There are giant cells in it. 



625 a (May 4, XIII). Hypodermic, eye-socket. Ulcer at base of eye. Typical giant cells, 

 but possibly foreign body giant cells. 



DO PLANTS HARBOR ANIMAL PARASITES? 



In a paper published in 1889 in the Comptes Rendus of the French Academy, Dr. 

 Domingos Freire undertakes to show that roses and various other common flowers harbor 

 bacteria, some of which are pathogenic to man. All this paper really proves is that which 



