CONTENTS 



Preface 3 



1. NOMENCLATURE AND CLASSIFICATION OF THE DIPH- 



THEROIDS 



Introduction 7 



HISTORICAL REVIEW OF DIPHTHEROIDS AND EMENDATIONS 8 



DISTRIBUTION OF DIPHTHEROIDS 18 



Diphtheroids in Hodgkin's Disease 17, 18 



Diphtheroids in Glands and Tissues 18 



Cultural Study and Grouping of Organisms 18, 19 



Diphtheroids from the Eye 21 



Nasal Diphtheroids 21 



Diphtheroids from Ascitic Fluids 21 



Diphtheroids in Blood Cultures 22 



Skin Diphtheroids 22 



Diphtheroids from the Brain 23 



Diphtheroids from the Appendix 23 



Source and Fermentative Reactions 24 



CLASSIFICATION OF THE UIPHTHEROIDS 25 



2. SEROLOGIC STUDY 



EXPERIMENT WITH HODGKIN'S STRAINS 30 



Agglutination Tests 31 



Complement Fixation 31 



APPLICATION OF AGGLUTININ ABSORPTION TESTS TO DIFFERENTIATION 



OF TYPE DIPHTHEROIDS 32 



RELATION OF TYPE DIPHTHEROIDS 33, 34 



3. PLEOMORPHISM OF DIPHTHEROIDS, MUTABILITY OF TYPES 



AND A METHOD FOR DETERMINING MUTANTS 



VARIABILITY OF BACTERIA 34 



CULTURAL STUDY OF MUTATION ." 36 



Experiments with Museum Strain of C. enzymicus (Mellon) 36 



Effect of Various Mediums on Selection of Type 37 



Experiments with Mellon Strain cultivated on Blood Medium.. 39 



STUDY WITH PURE LINE OF C. ENZYMICUS 39 



Bacillary Type 39 



"Transition" of Bacillus to Coccus 39 



Coccus Type 40 



Behavior of Coccus and Bacillary Types in Carbohydrates 41 



SEROLOGIC EXPERIMENTS 42 



Absorption of Agglutinins 42 



Absorption Experiments with Coccus Serum 42 



Absorption Experiments with Bacillary Serum 43 



SUMMARY AND CONCLUSIONS 44 



Bibliography 45. 46, 47 



Plates 1-3. Explanation of plates 48, 49, 51, 53 



Vita . . 55 



