48 FREDERICK EBERSON 



EXPLANATION OF PLATES 1-3 



Fig- 1. C. acidum. Large forms developing in mediums enriched with serum. X 1000. 

 Fig- 2. Same after prolonged cultivation on ordinary mediums. X 1000. 

 Fig. 3. C. suppuratum. On serum medium after 1st isolation. X 1000. 

 Fig- 4. Same after prolonged cultivation on ordinary medium. X 1000. 

 Fig. 5. C. aurantiacum. X 1000. 

 Fig. 6. C. delicatum. X 1000. 

 Fig. 7. C. epidermidis. X 1000. 

 Fig. 8. C. ascites. X 1000. 

 Fig. 9. C. glandulae. X 1000. 

 Fig. 10. C. pseudodiphtheriae. X 1000. 

 Fig. 11. C. cerebralis. X 1000. 

 Fig. 12. C. putidum. X 1000. 

 Fig. 13. C. flocculens. X 1000. 



Fig. 14. I. Plain agar slant inoculated with material taken from a culture of C. enzymicus 

 grown on sheep blood glucose agar for 4 generations. The sheep blood agar showed numerous 

 rod forms with cocci intermingled. A, 1 heavy white growth superimposed on a delicate trans- 

 parent background. 



only. Natural size. 



Fig. 15. Smear from heavy growth indicated by A 1 and B in Figure 14. 



Fig. 16. Smear from transparent delicate growth marked A in Figure 14. Stained with 

 Loeffler's methylene blue. X 1000. 



Fig. 17. C. enzymicus. Original culture. 



Fig.18. Bacillary type developing on Loeffler's serum. Transferred from sheep blood 

 agar on which the original culture had been inoculated. When the original strain (Fig. 17) 

 was planted on plain agar, bacilli could not be demonstrated. 



Fig. 19. Suppression of bacillary type on glucose agar. After 3 generations on Loeffler's 

 serum preceded by 2 generations on sheep blood agar, cocci were rare. Note the predom- 

 inating type here. 



Fig. 20. Transplant from 3rd generation on sheep blood medium to plain agar. The 

 sheep blood culture showed rare cocci and practically a pure culture of bacillary forms. 



Fig. 21. Same culture before transplanting to plain agar. X 1000. 



Fig. 22. C. enzymicus cultivated on blood agar, then transferred to plain agar and incu- 

 bated at 28 C. "Concentration of chromatin" clearly shown. 



Fig. 23. Transplant from 48-hour veal glucose broth culture of C. enzymicus. Note 

 absence of "transformed" bacilli. The technic according to Mellon. 



Fig. 24. C. enzymicus grown at 28 C. in broth. Curious chromatin staining. 



Fig. 25. C. enzymicus 3rd generation on agar from blood medium. This picture, as well 

 as Figure 22, is identical with the "transitional" phase. 



Fig. 26. C. enzymicus grown at 28 C. on plain agar for 10 days. Note the diplococcus 

 appearance. 



Fig. 27. Previous incubation at 28 C. in broth and subsequent growth at 37 C. Stained 

 according to Kinyoun's method. X 1000. 



