BACTERIOLOGICAL ANALYSES 309 



ing rim of the cover covered with a mixture of half paraffine and 

 half bee's wax. Pour into the pyrogallol in the desiccator 100 c.c 

 of water and then throw in 35 grams of potassium hydroxide. 

 Quickly insert culture tubes, or plates, and close the desiccator 

 with the cover, turning the cover so as to secure a perfect seal. 

 Apply three permanent screw clamps. 



Anaerobic germs of the type of Plectridium foetidum grow best 

 in freshly sterilized milk. In the case of Plectridium foetidum the 

 milk first curdles, then digests, forming a clear yellow liquid. The 

 digestion begins at the surface and proceeds downward. These 

 cultures develop a most penetrating foul odor, resembling that of 

 spoiled eggs. 1 



The technique and methods for determining the bacteriological 

 flora with reference to cultural and morphological characteristics of 

 individual species of microbes present, are identical to those used 

 in the bacteriological study of milk and other similar products, and 

 which are fully described in standard manuals on bacteriology. 



1 For further details on the technique of Anaerobic Cultures see Hunziker 

 Review of Existing Methods for Cultivating Anaerobic Bacteria. Journal of 

 Applied Microscopy and Laboratory Methods, Vol. V, Nos. 3, 4, 5, 6. 



