2O 



in every way identical with oxyurushin. The alkaline solutions 

 from which the oxyurushin was precipitated by acid, were evap- 

 orated and extracted with ether and alcohol but no organic sub- 

 stance was obtained. 



SEPARATION BY LEAD ACETATE AND SUBACETATE. 



Another part of the alcoholic residue, free from acetic acid, 

 was redissolved in alcohol and an alcoholic solution of lead ace- 

 tate added as long as it formed a precipitate. The 

 precipitate which was of a light gray color was washed with 

 alcohol, mixed with fresh alcohol, decomposed with sulphuric 

 acid, and the excess of acid removed by shaking with lead car- 

 bonate. On evaporating the alcohol a thick dark brown oily resi- 

 due was obtained. The residue was somewhat darker than the 

 original alcoholic residue, but otherwise similar. To the nitrate 

 secured from the lead acetate precipitate, lead subacetate was 

 added as long as a precipitate formed. The precipitate was of 

 a gray color, but a decidedly lighter gray than that obtained by 

 lead acetate. On decomposing the precipitate, as above, an oily 

 residue was obtained, which was also lighter in color than that 

 obtained from the lead acetate residue. 



The filtrate from the subacetate precipitate was still of a 

 brownish color. The excess of lead was removed by adding a 

 slight excess of sulphuric acid, and the excess of acid removed 

 by shaking with lead carbonate and filtering. The filtrate was 

 concentrated by evaporation and shaken out with ether. Upon 

 evaporating the ether a residue was obtained which, when dis- 

 solved in alcohol, was readily precipitated by lead acetate or sub- 

 acetate. By repeated experiments with the original alcoholic 

 solution it was found that by precipitation with lead acetate and 

 removing the lead and acid from the filtrate, reprecipitating and 

 continually repeating this operation, that a series of oily residues 

 could be obtained, gradually diminishing in quantity, and each 

 increasing in fluidity, and becoming a shade lighter than the pre- 

 ceding. Only the last fractions were poisonous. 



Lead subacetate is a better precipitant than the acetate. The 

 acetic acid liberated evidently aids in preventing complete pre- 

 cipitation. The fact that the fractions decrease in color and 

 viscosity, and that only the last were poisonous, indicates that the 

 alcoholic extract consists of a mixture of two or more substances. 



