The part soluble in methyl alcohol was soluble in each of the 

 above except benzin. The part insoluble in methyl alcohol but sol- 

 uble in ether, was insoluble in benzol, toluol, oxylol, alcohol, amyl 

 alcohol, carbon disulphide, turpentine, carbon tetrachloride, ace- 

 tic acid and chloral hydrate solution. 



The part insoluble in ether was also insoluble in any of the 

 above solvents. 



SEPARATION OF THE BENZIN-SOUJBLE PORTION. 



One volume of the substance was dissolved in eight volumes 

 of benzin, four volumes of alcohol added and the whole thor- 

 oughly agitated. Upon standing two layers appeared. The upper 

 benzin layer was of a yellowish brown color, the lower reddish 

 brown. These were separated and the benzin solution washed 

 with alcohol as long as any thing could be removed. The benzin 

 was driven off by evaporation leaving a non-poisonous, oily, 

 brown residue, insoluble in alcohol. 



The alcoholic solution was in turn washed by shaking out 

 with benzin but with no positive result. The solution was next 

 evaporated and a reddish brown, slightly gelatinous residue ob- 

 tained. By rapidly washing this with a small quantity of benzin 

 and evaporating a clear, light reddish brown residue was secured. 

 Both of these residues proved to be poisonous. By continual 

 washing with benzin or by employing it in larger quantities, the 

 entire residue was dissolved. I believe that this residue consists 

 of a poisonous and a non-poisonous substance but thus far the 

 separation has not been secured. I hope to be able to separate 

 them later. 



All of the resins separated from the lac were tested for 

 cholestrin but with negative results. 



CHEMICAL REACTIONS. 



All of the substances separated gave precipitates with lead 

 acetate, subacetate, silver nitrate, mercurous nitrate, cupric ace- 

 tate and ferric chloride. The lead precipitates were of a light 

 gray color gradually becoming darker on standing. The other 

 precipitates were black. All were slowly blackened by the action 

 of concentrated sulphuric acid. In the cold, concentrated nitric 

 acid colored the benzin-soluble substance red which changed to 

 brown on heating. The methyl alcohol-soluble substance was 



