26 



few hours. It was very hard to powder and was insoluble in all 

 ordinary solvents. When analyzed it gave the following results: 



0.3606 Gm. gave 0.295 Gm. H 2 O, 0.971 Gm. CO 2 

 0.4104 Gin. gave 7.3 Cc. at 25 C. N and 712 Mm. 



C 73430 

 9-J45 



N 1.850 



Ash 0.451 



A second sample of the above was dissolved in ether and 

 alcohol added, then precipitated with sodium hydrate, heated 

 for two hours on the steam bath, washed until free from alkali, 

 again heated for four hours w r ith hydrochloric acid, washed and 

 dried. Analysis gave the following result: 



0.2074 Gm. gave 0.194 Gm. H 2 O, 0.5482 Gin. CO 2 

 0.2926 Gm. gave 2.2 Cc. N at 21 C. and 712.5 Mm. 

 C 72.08 

 H 10.46 

 N 0.74 



Ash 1.02 



Part of the benzin-soluble substance was dissolved in alcohol, 

 a strong solution of sodium hydroxide added and heat applied. 

 An adhesive, black mass formed on the surface with a thick gray- 

 ish-black mixture underneath. When cold, globules of an oily sub- 

 stance could be seen. An attempt was made to separate them but 

 without success. Ether and benzin dissolved a large part of the 

 precipitate together with the oily globules, forming an insepar- 

 able emulsion. More sodium hydroxide was added and the whole 

 again heated for two hours, when a black precipitate separated 

 leaving a clear solution. The solution was separated as com- 

 pletely as possible by decantation. Distilled water was added 

 to the precipitate, when it again formed a homogeneous mixture 

 as inseparable as before but the addition of more alkali pro- 

 duced a separation. All attempts to free the precipitate from 

 alkali by washing failed, as it invariably formed the same homo- 

 geneous mixture which could not be filtered and refused to sep- 

 arate on standing. However, the addition of sodium chloride 

 to the mixture secured a perfect separation. The precipitate was 

 carefully collected on a filter and washed with solution of sodium 

 chloride until nearly free from alkali. It was then dried and 

 heated with hydrochloric acid which changed the color to reddish 

 brown. The precipitate was washed until free from acid, dried 

 and powdered. The powder was exhausted with ether which 



