49 



sist of aggregations of needles. This corresponds exactly with 

 the description and melting point given for phenylsorbinosazone. 80 

 A second crop of crystals was obtained by concentrating the moth- 

 er liquor. These were somewhat darker than the first and had a 

 M. P. of 157 C., but with the production of gas. This corres- 

 ponds with the description given for the osasone obtained from 

 the inactive sorbin. 



Furfurol was estimated from the gum according to Tollen's 

 method 81 and calculated as pentosan but the results were not sat- 

 isfactory. The method is designed for the estimation of furfurol 

 in food products, and has very little value for scientific investigation. 

 An analysis of the gum-enzyme gave the following results : 

 I 0.2626 Gm. gave 0.1424 H S O, 0.402 Gm. CO 2 . 

 II 0.3464 Gm. gave 0.1812 H 2 O, 0.529 Gm. CO 2 . 

 I 0.35 Gm. gave 2 cc. N at i8C. & 714.7 Mm. 

 II 0.452 Gm. gave 2.3 cc. N at i6C. & 713.7 Mm. 



I II MEAN 



C 4L742 41-645 -41.693 



H 6.067 S-58i 5.958 



N 0.630 0.587 0.608 



Ash 5.180 5.200 5.190 



O 46.551 



IOO.OOO 



Bertrand 82 when working with soluble oxidizing ferments 

 used the gum-enzyme from Japanese lac under the name "Lac- 

 case". He reports that it contained 0.44% of nitrogen which he 

 determined by heating with soda-lime and estimating the am- 

 monia formed by titrating with decinormal sulphuric acid. From 

 this he calculated the amount of enzyme present by assuming 

 that it has the elementary composition of albuminous substances. 



He then gives the composition of the gum-enzyme as : 



Water 74.00% 



Gum 84.95% 



Laccase 2 . 50% 



Ash S- 1 7% 



From the preceding work I think that I am justified in say- 

 ing that what he estimated as ammonia was not ammonia, but 

 pyrrol. 



80 Vaubel Quantitative Bestimmung. Organ. Verbindungen II, Band 

 3, 304. 



81 Lunge. II Bel. 460. 



8J Bull. Soc. Chim. 3 series, 51, p. 259, 1891. 



