258 APPENDIX. 



light. If the green plants are sought, exposure to direct 

 sunlight is advised. If Fungi are under cultivation, the 

 strong sunlight should be avoided. The light of almost 

 any ordinary room affords a sufficient variety of condi- 

 tions for nearly all sorts of cultivations, though some of 

 the Fungi may be raised to advantage in a damp cellar. 

 If the cultivations are being made during the summer 

 months, the temperature will take care of itself; in the 

 winter, to insure success, care must be taken that it does 

 not fall much below 70 F. at any time, day or night. 



In making the collections, not only should the different 

 known plants be placed in separate jars and the jars be filled 

 with water from the native habitat, but gatherings of mud 

 and debris from even unpromising localities should be util- 

 ized. Such unpromising collections often yield most sur- 

 prising and interesting results. For. instance, the finest 

 forms of that most fascinating organism Pandorina 

 which the writer has ever seen were developed in a jar 

 in which a clump of violets were kept with just moisture 

 sufficient to give vigorous growth to the violets. 



When rare plants or conjugating forms are secured, 

 some of them should be preserved in formalin or alcohol 

 to use in case subsequent cultures and search of the ponds 

 and streams fail to produce what is wanted. 



A box with glass front and back and a plate of glass for 

 a cover is very useful for the cultivation of Vaucheria, 

 Liverworts, Mosses, Drosera, and many other like plants. 

 It should stand in a window and should have three or four 

 inches of good swamp mud in the bottom. 



A large table, with a sink in its center provided with 

 running water, is also convenient for the cultivation of 

 many plants. Boxes of sawdust and earth arranged 

 around this table may be utilized for the production of 

 seedlings. Rich earth may be placed in the bottom of the 

 sink, and water plants may be grown. A small jet of 



