22 MICROSCOPY. 



The muscle of a salamander will be found excellent for demonstrat- 

 ing the structure of the fibers. 



Brain and Spinal Cord. Fix and harden in Muller's fluid or 

 Erlicki's fluid, two weeks for the former and fifteen days for the 

 latter. Wash thoroughly in water before embedding, which may be 

 done in paraffin. 



Heart. Fix in absolute alcohol; harden with alcohols; embed in 

 paraffin; stain with haematoxylin. 



Blood Vessels. Fix and harden with alcohol; embed in paraffin 

 or celloidin; and stain with lithium carmine or haematoxylin and 

 eosin. 



Lymphatic Glands. Fix and harden 1 with alcohol ; embed in cel- 

 loidin ; and stain with haematoxylin and eosin. 



Skin. Fix and harden with alcohol; embed in paraffin; and 

 stain with haematoxylin and eosin. 



The Spleen. Fix and harden with alcohol; embed in paraffin; 

 stain with haematoxylin and eosin. 



(Esophagus. Fix with corrosive subliniate or Perenyi's fluid; 

 harden with alcohol ; embed in paraffin ; and stain with haematoxy- 

 lin and eosin. 



Stomach. Fix with corrosive sublimate; harden with alcohol; 

 embed with paraffin or celloidin ; stain with haematoxylin. 



Intestine. Fix with corrosive sublimate; harden with alcohol; 

 embed in celloidin ; stain with hgematoxylin and eosin. 



Tongue. Fix with absolute alcohol ; harden with alcohols ; embed 

 in paraffin : and stain with haematoxylin and eosin. 



Trachea. After filling the trachea and lungs with a two-tenths 

 per cent solution of chromic acid, suspend them in a large volume of 

 the same for two days ; then cut into small pieces and place in two- 

 tenths per cent of chromic acid for two or three weeks ; wash thor- 

 oughly with water; embed in celloidin; and stain with haematoxylin. 



Lungs. These may be treated as the trachea; embed in par- 

 affin or celloidin; and stain with lithium carmine or haematoxylin 

 and eosin. 



Liver. Fix with corrosive sublimate, one per cent solution, twen- 

 ty-four hours; harden with alcohol; embed in paraffin: and stain 

 with hsematoxvlin and eosin. 



