MICROSCOPIC TECHNIQUE. 23 



Kidney. Fix and harden with alcohol; embed with paraffin; and 

 stain with haematoxylin and eosin. 



Ovary. Fix and harden with alcohol; embed in paraffin; and 

 stain with haematoxylin and eosin. The Fallopian tube may be 

 treated in the same way. 



Uterus. Fix and harden with alcohol; embed in paraffin; and 

 stain with haematoxylin and eosin. 



Testis. Fix with corrosive sublimate, and harden with alcohol; 

 embed in paraffin ; and stain with lithium carmine. 



Eye. Cut across the eye so as to partly divide it into an anterior 

 and posterior half; suspend in 150 cc. of chromic acid (0.25 per 

 cent) for twenty-four hours; sever the halves and renew the fluid; 

 after several days' wash in water, and harden in alcohol in the dark ; 

 embed in paraffin ; and stain with haematoxylin and eosin. 



Pancreas. Fix with Flemming's solution, twenty-four hours; 

 harden with alcohol ; stain with lithium carmine ; embedding may be 

 done in paraffin. 



III. STAINING METHODS. 



Sections to be stained may be fresh specimens or those that have 

 been cut from embedded tissue. They may be free that is, unat- 

 tached to the slide, or affixed. The following schemes for staining 

 are intended to be sufficiently comprehensive to include all of these 

 conditions : 



No, 1. METHOD FOR STAINING FRESH VEGETABLE 

 SECTIONS. 



( 1 ) Apply section to slide and add rosanilin violet, one to five min- 

 utes. 



(2) Wash in water to remove excess of stain. 



(3) Dry with blotting paper and add glycerine to dehydrate. 



(4) Remove excess of glycerine and add glycerine again to thor- 

 oughly dehydrate. 



(5) Wipe off excess of glycerine and add xylol twice. 



(6) Apply to cover-glass a drop or two of xylol-balsam, and, hav- 

 ing wiped off the excess of xylol from the slide, drop it gently (bal- 

 sam down) upon the section. Then apply gentle pressure with dis- 

 secting needle to spread out the balsam. 



