24: MICROSCOPY. 



(7) Label and keep in a horizontal position until the balsam is 

 hardened. 



Vegetable Sections. To stain paraffin or celloidin sections of 

 plant structures, the methods are practically the same as those given 

 below for animal objects. 



Methods for Animal Sections. 



No. 2. CARMINE METHOD FOR FREE SECTIONS. 



(1) Apply section to slide and wash with thirty-five per cent al- 

 cohol. 



(2) Add lithium carmine sufficient to cover section., one to five 

 minutes. 



(3) If necessary, remove excess of stain with acid alcohol, five 

 to ten seconds. 



(4) Dehydrate with increasing strengths of alcohol thirty-five 

 per cent, seventy-five per cent, ninety-five per cent, and absolute. 



(5) Wipe off excess of alcohol, and when section is partly dry 

 add creosote to clear up, five to ten minutes. 



(6) Wipe off excess of creosote and mount with balsam. 



(7) Center cover-glass and apply pressure to spread out the bal- 

 sam. 



(8) Label and lay aside in horizontal position, cover-glass up, 

 until the balsam hardens. 



No. 3. CARMINE METHOD WITH AFFIXED PARAFFIN 



SECTIONS. 



(1) Apply to the center of the slide a thin layer of collodion- 

 clove-oil mixture. 



(2) Center and attach the section, applying the heat of a spirit 

 or Bunsen flame. 



(3) Immerse in xylol two minutes and in turpentine ten min- 

 utes to remove paraffin. Sections immersed in turpentine alone 

 should remain twenty minutes. 



(4) Wash with alcohol, decreasing strengths, using thirty-five 

 per cent alcohol last. 



