MICROSCOPIC TECHNIQUE. 25 



(5) Apply lithium carmine, one to ten minutes. 



(6) Remove excess of stain with acid alcohol. 



(7) Dehydrate with alcohol, increasing strengths. 



(8) Dry and clear up with creosote, five to ten minutes. 



(9) Wipe off excess of creosote and mount in balsam. 



(10) Center cover-glass. 



(11) Label and lay aside in horizontal position until balsam 

 hardens. 



No. 4. CARMINE METHOD FOR AFFIXED CELLOIDIN 

 SECTIONS. 



(1) Center section and affix with collodion mixture. 



(2) Stain with lithium carmine, one to five minutes. 



(3) Remove excess of stain with acid alcohol, five to ten seconds. 



(4) Apply seventy per cent alcohol. 



(5) Apply eighty per cent alcohol. 



(6) Apply ninety-five per cent alcohol a few seconds. 



(7) Clear up with creosote. 



(8) Remove excess of creosote with blotting paper. 



(9) Mount with balsam and center cover-glass. 



(10) Label and lay aside in a horizontal position. 



No. 5. H^IMATOXYLIN METHOD FOR FREE SECTIONS. 



(1) With section on slide, apply alcohol of the same strength as 

 staining solution. , 



(2) Stain with diluted ha3matoxylin, one to ten minutes. 



(3) Remove excess of stain with thirty-five per cent alcohol. 



(4) Dehydrate with alcohols, increasing strengths. 



(5) Clear up with creosote or cedar oil. 



(6) Center section and apply balsam and cover-glass. 



(7) Center cover-glass. 



(8) Label and lay aside in horizontal position until balsam 

 hardens. 



No. 6. KffiMATOXYLIN METHOD FOR AFFIXED PARAF- 

 FIN SECTIONS. 



(1) Apply to slide a thin layer of egg-albumen and glycerine. 



