26 MICROSCOPY. 



(2) Center section and flatten it by gently touching with end of 

 finger. 



(3) Apply heat of flame until paraffin melts (sections that have 

 been flattened upon water should be heated much longer than 

 others). 



(4) Eemove paraffin with xylol or turpentine. 



(5) Eemove xylol, etc., with alcohol, decreasing strengths. 



(6) Stain with diluted haematoxylin, one to ten minutes. 



(7) Remove excess of stain with thirty-five per cent alcohol. 



(8) Dehydrate with alcohol. 



(9) Clear up with creosote, five to ten minutes. 



(10) Wipe off excess of creosote and mount with balsam. 



(11) Center cover-glass. 



(12) Label and keep in horizontal position until balsam hardens. 



No. 7. HJEMATOXYLIN METHOD FOE AFFIXED CELLOIDIN 



SECTIONS, 



(1) Center section and affix with collodion mixture. 



(2) Stain with diluted hgematoxylin, one to ten minutes. 



(3) Remove excess of stain with acid alcohol. 



(4) Apply seventy per cent alcohol. 



(5) Apply eighty per cent alcohol. 



(6) Apply ninety-five per cent alcohol a few seconds. 



(7) Clear up with creosote, five to ten minutes. 



(8) Remove excess of creosote with blotting paper. 



(9) Mount with balsam and center cover-glass. 



(10) Label and lay aside in a horizontal position until balsam 

 is hardened. 



No. 8. KEMATOXYLIN-EOSIN METHOD. 



( 1 ) If desired, affix section to slide with egg-albumen and glyc- 

 erine or collodion and clove-oil. 



(2) If a paraffin section, remove paraffin with xylol or turpen- 

 tine or both; remove xylol, etc., with alcohol. 



(3) Apply thirty-five per cent alcohol. 



(4) Stain with diluted hsematoxylin, one to five minutes. 



(5) Apply thirty-five per cent alcohol to remove excess of stain. 



