REAGENTS AND STAINS. 31 



A good reagent for embryos and adult tissues. Time, three to 

 twelve hours ; dehydrate with alcohol. 



Erlicki's fluid 



Potassium bi-chromate 2.5 grams. 



Cupric sulphate 0.5 grams. 



Water 100 cc. 



A good reagent for general use, for embryos and nervous tissues. 

 Time, ten to fifteen days ; dehydrate with alcohol. 



Corrosive sublimate 



Aqueous Solution 



Corrosive sublimate 1 gram. 



Water 95 cc. 



Alcoholic Solution 



Corrosive sublimate 1 gram. 



Alcohol (ninety-five per cent) ...... .99 cc. 



Used for general purposes, and specially for alimentary tract. 

 Time, twenty-four hours, hardening in alcohol, to which a few 

 crystals of iodine have been added. 



Chromic acid. Use a 0.5 per cent solution, dehydrating with 

 alcohol in the dark. 



Muller's fluid- 

 Potassium bi-chromate 25 grams. 



Sodium sulphate 10 grams. 



Water 1,000 cc. 



Pulverize the solids before adding water, and use a piece of cam- 

 phor in the solution to prevent the formation of fungi. Good for 

 general use and especially valuable for central nervous system. Re- 

 quires from two to six weeks. Wash in water for several days, and 

 dehydrate with alcohol. 



Flemming's fluid 



Chromic acid (one per cent solution) ... .46 cc. 



Osmic acid (two per cent solution) ...... 12 cc. 



Glacial acetic acid.. . 3 cc. 



