REAGENTS AND STAINS. 33 



Hanstem's rosanilin violet 



Methyl violet 1 gram. 



Fuchsia 1 gram. 



Distilled water 100 cc. 



Note. This is a valuable stain for vegetable sections. It should 

 be diluted for use as desired. 



Lithium carmine 



Carmine 2.5 grams. 



Lithium carbonate (saturated solu- 

 tion) 100 cc. 



The carmine should be dissolved in cold solution. Sections stain 

 rapidly, and should be decolorized with acid alcohol. 



Dalafield's haematoxylin 



1. Hasmatoxylin 1 gram. 



2. Absolute alcohol 6 cc. 



3. Ammonia alum (saturated sol.)... 100 cc. 



4. Glycerine 25 cc. 



5. Methyl alcohol 25 cc. 



Process. Dissolve (1) in (2) ; add this solution to (3) ; expose 

 to air and light for a week; filter and add (4) and (5) ; allow it to 

 stand for a long time exposed to air and light. 



Eosin 



Alcoholic Eosin for Sections. Make a saturated alcoholic solu- 

 tion. This is used as a ground stain in connection with haematoxy- 

 lin; also as a blood stain. 



Magenta for hlood, etc. 



Magenta . 1 gram. 



Alcohol (eighty-five per cent) 50 cc. 



Water 150 cc. 



Glycerine 200 cc. 



Methylene blue for hlood 

 Make a saturated aqueous solution. 



