34 MICROSCOPY. 



Carter's carmine mass for injecting 



Carmine 3 grams. 



Strong ammonia 6 cc. 



Glacial acetic acid 6 cc. 



Coignet's French gelatin 7 grams. 



Water 80 cc. 



Process. " Place the finely cut gelatin in 50 cc. of water for five 

 hours ; dissolve the carmine in a mortar with a little water, and add 

 the ammonia; let stand for two hours and then pour into a bottle, 

 rinsing the mortar with the remainder of water; place the gelatin 

 and water on a water-bath until the gelatin melts. To the carmine 

 fluid add the acetic acid, a few drops at a time (rinsing mortar thor- 

 oughly) until the fluid becomes crimson. To the melted gelatin add 

 the crimson carmine, little by little, with continual stirring. Keep 

 in a cool place with surface covered with methylated spirit. When 

 wanted for use, dissolve on water-bath and filter through flannel 

 wrung out of hot water." (Fearnley's Method.) 



CLEARING AGENTS. 



Those commonly used are cedar oil, creosote, clove-oil, xylol, and 

 aniline oil. Clove-oil cannot be used with celloidin sections. 



MOUNTING MEDIA. 



Glycerine jelly and Canada balsam are commonly used for mount- 

 ing purposes. For the laboratory balsam will be found a satisfactory 

 medium. Should xylol be used for clearing, the balsam should be 

 dissolved in xylol. Chloroform balsam may be used in sections cleared 

 with chloroform. 



For the formulae of reagents and stains required for work in bac- 

 teriology and urinalysis, the reader is referred to the chapters in 

 which is discussed the micro-technique of these subjects. 



